DNAJB1 Antikörper (PE)
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- Target Alle DNAJB1 Antikörper anzeigen
- DNAJB1 (DnaJ (Hsp40) Homolog, Subfamily B, Member 1 (DNAJB1))
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Reaktivität
- Human
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Wirt
- Maus
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Klonalität
- Monoklonal
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Konjugat
- Dieser DNAJB1 Antikörper ist konjugiert mit PE
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Applikation
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF), Immunocytochemistry (ICC)
- Spezifität
- Detects ~40 kDa. Does not cross-react with HDJ2 or YDJ1.
- Kreuzreaktivität
- Human, Maus, Ratte
- Aufreinigung
- Protein G Purified
- Immunogen
- Recombinant Protein HSP40 (Hdj1)
- Klon
- 3B9-E6
- Isotyp
- IgG1
- Top Product
- Discover our top product DNAJB1 Primärantikörper
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- Applikationshinweise
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- WB (1:2000)
- ICC/IF (1:100)
- optimal dilutions for assays should be determined by the user.
- Kommentare
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0.5 μg/ml of ABIN2481902 was sufficient for detection of HSP40 (HDJ1) in 15 μg of HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Konzentration
- 1 mg/mL
- Buffer
- PBS pH 7.2, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Lagerung
- 4 °C
- Informationen zur Lagerung
- Conjugated antibodies should be stored at 4°C
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- Target
- DNAJB1 (DnaJ (Hsp40) Homolog, Subfamily B, Member 1 (DNAJB1))
- Andere Bezeichnung
- HSP40 (DNAJB1 Produkte)
- Synonyme
- HSPF1 antikoerper, Hdj1 antikoerper, Hsp40 antikoerper, RSPH16B antikoerper, Sis1 antikoerper, CG10578 antikoerper, DNAJ-1 antikoerper, DNAJ1 antikoerper, DROJ1 antikoerper, Dmel\\CG10578 antikoerper, DnaJ1 64EF antikoerper, DroJ1 antikoerper, EU3500 antikoerper, HSP40 antikoerper, HSP40/HDJ1 antikoerper, Hsp-40 antikoerper, anon-WO0140519.166 antikoerper, anon-WO0172774.135 antikoerper, dHDJ1 antikoerper, dHDJ1/HSP40 antikoerper, dHdj1 antikoerper, dhdJ1 antikoerper, dhdj-1 antikoerper, dhdj1 antikoerper, dnaJ-1 antikoerper, droj1 antikoerper, hsp40 antikoerper, dnajb4 antikoerper, hdj1 antikoerper, hspf1 antikoerper, sis1 antikoerper, DnaJ-5 antikoerper, MAS5 antikoerper, dnaJ antikoerper, 0610007I11Rik antikoerper, dnajb1 antikoerper, zf-Hsp40 antikoerper, zgc:101068 antikoerper, DnaJ heat shock protein family (Hsp40) member B1 antikoerper, DnaJ-like-1 antikoerper, DnaJ (Hsp40) homolog 5 antikoerper, type I HSP40 co-chaperone YDJ1 antikoerper, DnaJ protein antikoerper, molecular chaperone DnaJ antikoerper, DnaJ (Hsp40) homolog, subfamily B, member 1a antikoerper, DNAJB1 antikoerper, Dnajb1 antikoerper, DnaJ-1 antikoerper, dnajb1 antikoerper, DnaJ-5 antikoerper, YDJ1 antikoerper, dnaJ antikoerper, dnajb1a antikoerper
- Hintergrund
- Human HSP40/DnaJ proteins comprise a large protein family, members of which feature the J domain (named after the bacterial DnaJ protein) (1). The J-domain spans the first 75 N-terminal amino acids and is separated from the C-terminal by a glycine/phenylalanine-rich domain (2). Members of the HSP40/DnaJ family play diverse roles in many cellular processes, such as folding, translocation, degradation and assembly of multi-protein complexes. In particular, Hdj1, the first human HSP40/DnaJ protein identified, plays an important role in protein translation and folding, as well as in the regulation of HSP70 function (3). HSP40 stimulates the ATPase activity of HSP70 which in turn causes conformational changes of the unfolded proteins (4, 5). The HSP40-HSP70-unfolded protein complex further binds to co-chaperones Hip, Hop and HSP90 which leads to protein folding, or components of protein degradation machinery CHIP and BAG-1 (6). Some studies have shown that the difference between HDJ1 and type 1 DNAJ proteins including HDJ2 and yeast YdjI is the result of the possession of a zinc finger domain by the latter, which helps in the function of protein folding. (7, 8).
- Gen-ID
- 3337
- NCBI Accession
- NP_006136
- UniProt
- P25685
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