HSPA4 Antikörper (Atto 488)

Produktnummer ABIN2481905

Produktdetails anti-HSPA4 Antikörper (Atto 488)

Target: HSPA4 (Heat Shock 70kDa Protein 4 (HSPA4))

Reaktivität: Hamster

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser HSPA4 Antikörper ist konjugiert mit Atto 488

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Spezifität: Detects ~110 kDa.

Kreuzreaktivität: Rind (Kuh), Hamster, Human, Affe, Maus, Ratte, Saccharomyces cerevisiae, Hai, Schaf

Aufreinigung: Protein A Purified

Immunogen: Synthetic peptide derived from the sequence of hamster HSP110, sequence identical to human and mouse

Anwendungsinformationen

Applikationshinweise:

• WB (1:1000)
• IHC (1:100)
• optimal dilutions for assays should be determined by the user.

Kommentare:

1 μg/ml of ABIN2481905 was sufficient for detection of HSP110 in 10 μg of human cell line mixed lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: PBS pH 7.2, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Conjugated antibodies should be stored at 4°C

Details zu HSPA4

Target: HSPA4 (Heat Shock 70kDa Protein 4 (HSPA4))

Andere Bezeichnung: HSP110 (HSPA4 Produkte)

Synonyme: APG-2 antikoerper, HS24/P52 antikoerper, HSPH2 antikoerper, RY antikoerper, hsp70 antikoerper, hsp70RY antikoerper, Hsp110 antikoerper, Hsp70 antikoerper, irp94 antikoerper, 70kDa antikoerper, AI317151 antikoerper, Hsp70RY antikoerper, mKIAA4025 antikoerper, hspa4 antikoerper, wu:fi30e11 antikoerper, zgc:55743 antikoerper, zgc:77413 antikoerper, hs24/p52 antikoerper, hspa4-a antikoerper, osp94 antikoerper, pg-2 antikoerper, hspa4l antikoerper, wu:fc41d05 antikoerper, wu:fi59h02 antikoerper, wu:fj35c08 antikoerper, zgc:55506 antikoerper, heat shock protein family A (Hsp70) member 4 antikoerper, heat shock protein family A member 4 antikoerper, heat shock protein 4 antikoerper, heat shock protein 4b antikoerper, heat shock protein family A (Hsp70) member 4 S homeolog antikoerper, heat shock protein 4a antikoerper, HSPA4 antikoerper, Hspa4 antikoerper, hspa4b antikoerper, hspa4.S antikoerper, hspa4a antikoerper

Hintergrund: HSP110 belongs to a family of large stress proteins known as the HSP110/SSE Family. The proteins in this family are the most distantly known relatives of the well studied HSP70 family. They share 30-33 % amino acid identity, mostly in the conserved ATP-binding domain (1). HSP110 cooperates with HSP70 in protein folding in the eukaryotic cytosol (2). In mammals, HSP110 is constitutively expressed, but exhibits particularly high levels in the brain. Both HSP70 and HSP110 are elevated after cerebral ischemia. Recent studies demonstrate that the protective effects of HSP110 deficiency in cerebral ischemia may partly be mediated by an increase in the chaperone activity of HSP70 (3). Studies also suggest that HSP110 can be used in heat shock protein-based cancer immunotherapy (4).

Gen-ID: 10808

NCBI Accession: NP_006635

UniProt: Q92599