Biliverdin Reductase Antikörper (Biotin)

Produktnummer ABIN2482108

Produktdetails anti-Biliverdin Reductase Antikörper (Biotin)

Target: Biliverdin Reductase (BLVRA) (Biliverdin Reductase A (BLVRA))

Reaktivität: Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Biliverdin Reductase Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)

Spezifität: Detects ~36 kDa.

Kreuzreaktivität: Human, Maus, Ratte

Aufreinigung: Protein A Purified

Immunogen: Rat native full-length BVR purified from liver tissue

Anwendungsinformationen

Applikationshinweise:

• WB (1:500)
• IHC (1:1000)
• IP (1:100)
• optimal dilutions for assays should be determined by the user.

Kommentare:

2 μg/ml of ABIN2482108 was sufficient for detection of BVR in 20 μg of mixed human cell line lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Conjugated antibodies should be stored at 4°C

Details zu Biliverdin Reductase

Target: Biliverdin Reductase (BLVRA) (Biliverdin Reductase A (BLVRA))

Andere Bezeichnung: BVR (BLVRA Produkte)

Synonyme: blvra antikoerper, MGC69310 antikoerper, BLVRA antikoerper, zgc:153046 antikoerper, BVR antikoerper, Dmel\\CG9471 antikoerper, BLVR antikoerper, BVRA antikoerper, 0610006A11Rik antikoerper, 2500001N03Rik antikoerper, Blvr antikoerper, biliverdin reductase A antikoerper, biliverdin reductase A L homeolog antikoerper, CG9471 gene product from transcript CG9471-RA antikoerper, BLVRA antikoerper, blvra antikoerper, blvra.L antikoerper, CG9471 antikoerper, Blvra antikoerper

Hintergrund: Biliverdin Reductase (BVR) is a cytoplasmic enzyme that catalyzes the conversion of biliverdin to bilirubin by converting a double bond between the second and third pyrrole ring into a single bond (1). It is ubiqutiously expressed in all tissues- it occurs in cells and brain regiuons that already display HO-1 and HO-2, but also in regions and cell types with potential to induce stress proteins. It is unique among all enzymes in having two pH optima, using a different cofactor at each pH range, NADH at pH 7.0 and NADPH at pH 8.7 (2). It is not inactivated by heat shock, and have shown to abate inflammation, oxidative stress and apoptosis (3).

Gen-ID: 116599

NCBI Accession: NP_446302

UniProt: P46844