Notch1 Antikörper (AA 20-216) (FITC)
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- Target Alle Notch1 (NOTCH1) Antikörper anzeigen
- Notch1 (NOTCH1) (Notch 1 (NOTCH1))
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Bindungsspezifität
- AA 20-216
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Reaktivität
- Maus
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Wirt
- Maus
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Klonalität
- Monoklonal
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Konjugat
- Dieser Notch1 Antikörper ist konjugiert mit FITC
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Applikation
- Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)
- Spezifität
- Detects >270 kDa. Does not cross-react with Notch 2 or Notch3.
- Kreuzreaktivität
- Human, Maus, Ratte
- Aufreinigung
- Protein G Purified
- Immunogen
- Fusion protein amino acids 20-216 (extracellular N-terminus, EGF-like domains 1-5) of mouse Notch1
- Klon
- S253-32
- Isotyp
- IgG1
- Top Product
- Discover our top product NOTCH1 Primärantikörper
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- Applikationshinweise
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- WB (1:1000)
- optimal dilutions for assays should be determined by the user.
- Kommentare
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1 μg/ml of ABIN2483200 was sufficient for detection of Notch1 in 20 μg of rat brain membrane lysate and assayed by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Konzentration
- 1 mg/mL
- Buffer
- PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Lagerung
- 4 °C
- Informationen zur Lagerung
- Conjugated antibodies should be stored at 4°C
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- Target
- Notch1 (NOTCH1) (Notch 1 (NOTCH1))
- Andere Bezeichnung
- Notch1 (NOTCH1 Produkte)
- Synonyme
- notch1 antikoerper, NOTCH1 antikoerper, hn1 antikoerper, tan1 antikoerper, notch antikoerper, xotch antikoerper, xnotch antikoerper, notch-1 antikoerper, xnotch1 antikoerper, TAN1 antikoerper, hN1 antikoerper, 9930111A19Rik antikoerper, Mis6 antikoerper, N1 antikoerper, Tan1 antikoerper, lin-12 antikoerper, NOTCH antikoerper, Xotch antikoerper, notch1-a antikoerper, notch 1 antikoerper, fibropellin-1 antikoerper, Notch homolog 1, translocation-associated (Drosophila) antikoerper, Notch homolog 1, translocation-associated antikoerper, notch 1 S homeolog antikoerper, notch1 antikoerper, LOC100631526 antikoerper, NOTCH1 antikoerper, Notch1 antikoerper, notch1.S antikoerper
- Hintergrund
- Notch is synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase (S1 cleavage) in the trans-golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved (S2 cleavage) by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called Notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin-dependent gamma-secretase (S3 cleavage) to release the intracellular domain (NICD) from the membrane.
- Gen-ID
- 18128
- NCBI Accession
- NP_032740
- UniProt
- Q01705
- Pathways
- Notch Signalweg, Stem Cell Maintenance, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development
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