LAMP2 Antikörper (Atto 390)

Produktnummer ABIN2484600

Produktdetails anti-LAMP2 Antikörper (Atto 390)

Target: LAMP2 (Lysosomal-Associated Membrane Protein 2 (LAMP2))

Reaktivität: Maus

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser LAMP2 Antikörper ist konjugiert mit Atto 390

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunofluorescence (IF)

Spezifität: Detects ~100-110 kDa.

Kreuzreaktivität: Human, Maus, Kaninchen

Aufreinigung: Protein G Purified

Immunogen: Purified preparation of mouse liver lysosomal membranes

Klon: GL2A7

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise:

• WB (1:1000)
• ICC/IF (1:500)
• optimal dilutions for assays should be determined by the user.

Kommentare:

1 μg/ml of ABIN2484600 was sufficient for detection of LAMP2 in 20 μg of rat liver microsomes by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Conjugated antibodies should be stored at 4°C

Details zu LAMP2

Target: LAMP2 (Lysosomal-Associated Membrane Protein 2 (LAMP2))

Andere Bezeichnung: LAMP2 (LAMP2 Produkte)

Synonyme: CD107b antikoerper, LAMP-2 antikoerper, LAMPB antikoerper, LGP110 antikoerper, Lamp-2 antikoerper, Lamp-2a antikoerper, Lamp-2b antikoerper, Lamp-2c antikoerper, Mac3 antikoerper, cd107b antikoerper, lamp-2 antikoerper, lampb antikoerper, LAMP2 antikoerper, lamp2 antikoerper, DKFZp459J126 antikoerper, LGP-B antikoerper, sb:cb587 antikoerper, wu:fb14a06 antikoerper, zgc:103532 antikoerper, lysosomal associated membrane protein 2 antikoerper, lysosomal-associated membrane protein 2 antikoerper, lysosomal-associated membrane protein 2 S homeolog antikoerper, lysosomal membrane glycoprotein 2 antikoerper, LAMP2 antikoerper, Lamp2 antikoerper, lamp2.S antikoerper, lamp2 antikoerper

Hintergrund: Lysosme associated membrane proteins, or LAMP1 and LAMP2, are major constituents of the lysosomal membrane. The two have closely related structures, with 37 % sequence homology (2). They are both transmembrane glycoproteins that are localized primarily in lysosomes and late endosomes. Newly synthesized molecules are mostly transported from the trans-Golgi network directly to endosomes and then to lysosomes. A second pathway involves the lamps being delivered from the Golgi to the cell surface, and then along the endocytic pathway to the lysosomes. A minor pathway involves transport via the plasma membrane (3). LAMP2 has also been detected at the plasma membrane of cells, as well as in cells that secrete lysosomal hydrolases. A study in the developmental expresses patterns of membrane LAMP2 transcripts indicate a possible involvement of this protein in cell-cell or cell-extracellular matrix interaction, and appear to reflect tissue and cell type specific roles of lysosomes during morphogenesis (4). Upon stimulation, a rapid translocation of intracellular LAMPs to the cell membrane is dependent on a carboxyl-terminal tyrosine based motif (YXXI) (5). This stimulation has also been shown to have an associated release of histamine, leukotriene C4 and prostaglandin D2, which shows that LAMP1 and LAMP2 are activation markers for normal mast cells (5). They have also been linked to the inflammatory response in that they promote adhesion of human peripheral blood mononuclear cells (PBMC) to vascular endothelium, and therefore possibly the adhesion of PBMC to the site of inflammation (6). LAMP2 has also been shown to be critical for autophagy, in conversion of early autophagic vacuoles to vacuoles which rapidly degrade their content (7).

Gen-ID: 16784

NCBI Accession: NP_001017959

UniProt: P17047

Pathways: Autophagie