HSF1 Antikörper (AA 378-395) (Atto 488)

Produktnummer ABIN2484618

Produktdetails anti-HSF1 Antikörper (Atto 488)

Target: HSF1 (Heat Shock Factor Protein 1 (HSF1))

Bindungsspezifität: AA 378-395

Reaktivität: Maus

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser HSF1 Antikörper ist konjugiert mit Atto 488

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF), Gel Shift (GS), Immunocytochemistry (ICC)

Spezifität: Detects ~85 kDa (unstressed cell lysates), and~95 kDa (heat shocked cell lysates).

Kreuzreaktivität: Rind (Kuh), Meerschweinchen, Hamster, Human, Affe, Maus, Kaninchen, Ratte

Aufreinigung: Protein G Purified

Immunogen: Purified recombinant mouse HSF1 protein, with epitope mapping to amino acids 378-395

Klon: 10H8

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise:

• WB (1:1000)
• IHC (1:1000)
• ICC/IF (1:200)
• optimal dilutions for assays should be determined by the user.

Kommentare:

1 μg/ml of ABIN2484618 was sufficient for detection of HSF1 in 20 μg of heat shocked HeLa cell lysate by ECL immunoblot analysis using Goat anti-rat IgG: HRP as the secondary antibody

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Conjugated antibodies should be stored at 4°C

Details zu HSF1

Target: HSF1 (Heat Shock Factor Protein 1 (HSF1))

Andere Bezeichnung: HSF1 (HSF1 Produkte)

Synonyme: hsf1 antikoerper, Hsf antikoerper, xhsf1 antikoerper, AA960185 antikoerper, HSTF1 antikoerper, HSF1 antikoerper, HSF antikoerper, HSF1c antikoerper, hsf1a antikoerper, hsf1b antikoerper, zHSF1a antikoerper, zHSF1b antikoerper, hstf1 antikoerper, heat shock factor antikoerper, Heat Shock Factor antikoerper, heat shock factor 1 antikoerper, heat shock transcription factor 1 antikoerper, heat shock transcription factor 1 S homeolog antikoerper, hsf1 antikoerper, hsf-1 antikoerper, Hsf1 antikoerper, HSF1 antikoerper, hsf1.S antikoerper

Hintergrund: HSF1, or heat shock factor 1, belongs to a family of Heat Shock transcription factors that activate the transcription of genes encoding products required for protein folding, processing, targeting, degradation, and function (2). The up-regulation of HSP (heat shock proteins) expression by stressors is achieved at the level of transcription through a heat shock element (HSE) and a transcription factor (HSF) (3, 4, 5). Most HSFs have highly conserved amino acid sequences. On all HSFs there is a DNA binding domain at the N-terminus. Hydrophobic repeats located adjacent to this binding domain are essential for the formation of active trimers. Towards the C-terminal region another short hydrophobic repeat exists, and is thought to be necessary for suppression of trimerization (6). There are two main heat shock factors, 1 and 2. Mouse HSF1 exists as two isoforms, however in higher eukaryotes HSF1 is found in a diffuse cytoplasmic and nuclear distribution in un-stressed cells. Once exposed to a multitude of stressors, it localizes to discrete nuclear granules within seconds. As it recovers from stress, HSF1 dissipates from these granules to a diffuse nuceloplasmic distribution. HSF2 on the other hand is similar to mouse HSF1, as it exists as two isoforms, the alpha form being more transcriptionally active than the smaller beta form (7, 8). Various experiments have suggested that HFS2 may have roles in differentiation and development (9, 10, 11).

Gen-ID: 15499

NCBI Accession: NP_032322

UniProt: P38532