HSF1 Antikörper (AA 378-395) (PerCP)
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- Target Alle HSF1 Antikörper anzeigen
- HSF1 (Heat Shock Factor Protein 1 (HSF1))
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Bindungsspezifität
- AA 378-395
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Reaktivität
- Maus
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Wirt
- Ratte
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Klonalität
- Monoklonal
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Konjugat
- Dieser HSF1 Antikörper ist konjugiert mit PerCP
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Applikation
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF), Gel Shift (GS), Immunocytochemistry (ICC)
- Spezifität
- Detects ~85 kDa (unstressed cell lysates), and~95 kDa (heat shocked cell lysates).
- Kreuzreaktivität
- Rind (Kuh), Meerschweinchen, Hamster, Human, Affe, Maus, Kaninchen, Ratte
- Aufreinigung
- Protein G Purified
- Immunogen
- Purified recombinant mouse HSF1 protein, with epitope mapping to amino acids 378-395
- Klon
- 10H8
- Isotyp
- IgG1
- Top Product
- Discover our top product HSF1 Primärantikörper
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- Applikationshinweise
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- WB (1:1000)
- IHC (1:1000)
- ICC/IF (1:200)
- optimal dilutions for assays should be determined by the user.
- Kommentare
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1 μg/ml of ABIN2484631 was sufficient for detection of HSF1 in 20 μg of heat shocked HeLa cell lysate by ECL immunoblot analysis using Goat anti-rat IgG: HRP as the secondary antibody
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Konzentration
- 1 mg/mL
- Buffer
- PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Lagerung
- 4 °C
- Informationen zur Lagerung
- Conjugated antibodies should be stored at 4°C
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- Target
- HSF1 (Heat Shock Factor Protein 1 (HSF1))
- Andere Bezeichnung
- HSF1 (HSF1 Produkte)
- Synonyme
- hsf1 antikoerper, Hsf antikoerper, xhsf1 antikoerper, AA960185 antikoerper, HSTF1 antikoerper, HSF1 antikoerper, HSF antikoerper, HSF1c antikoerper, hsf1a antikoerper, hsf1b antikoerper, zHSF1a antikoerper, zHSF1b antikoerper, hstf1 antikoerper, heat shock factor antikoerper, Heat Shock Factor antikoerper, heat shock factor 1 antikoerper, heat shock transcription factor 1 antikoerper, heat shock transcription factor 1 S homeolog antikoerper, hsf1 antikoerper, hsf-1 antikoerper, Hsf1 antikoerper, HSF1 antikoerper, hsf1.S antikoerper
- Hintergrund
- HSF1, or heat shock factor 1, belongs to a family of Heat Shock transcription factors that activate the transcription of genes encoding products required for protein folding, processing, targeting, degradation, and function (2). The up-regulation of HSP (heat shock proteins) expression by stressors is achieved at the level of transcription through a heat shock element (HSE) and a transcription factor (HSF) (3, 4, 5). Most HSFs have highly conserved amino acid sequences. On all HSFs there is a DNA binding domain at the N-terminus. Hydrophobic repeats located adjacent to this binding domain are essential for the formation of active trimers. Towards the C-terminal region another short hydrophobic repeat exists, and is thought to be necessary for suppression of trimerization (6). There are two main heat shock factors, 1 and 2. Mouse HSF1 exists as two isoforms, however in higher eukaryotes HSF1 is found in a diffuse cytoplasmic and nuclear distribution in un-stressed cells. Once exposed to a multitude of stressors, it localizes to discrete nuclear granules within seconds. As it recovers from stress, HSF1 dissipates from these granules to a diffuse nuceloplasmic distribution. HSF2 on the other hand is similar to mouse HSF1, as it exists as two isoforms, the alpha form being more transcriptionally active than the smaller beta form (7, 8). Various experiments have suggested that HFS2 may have roles in differentiation and development (9, 10, 11).
- Gen-ID
- 15499
- NCBI Accession
- NP_032322
- UniProt
- P38532
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