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HSF1 Antikörper (AA 378-395) (PerCP)

HSF1 Reaktivität: Maus WB, ELISA, IHC, IP, IF, GS, ICC Wirt: Ratte Monoclonal 10H8 PerCP
Produktnummer ABIN2484631
  • Target Alle HSF1 Antikörper anzeigen
    HSF1 (Heat Shock Factor Protein 1 (HSF1))
    Bindungsspezifität
    • 30
    • 15
    • 13
    • 12
    • 11
    • 10
    • 9
    • 9
    • 7
    • 7
    • 7
    • 6
    • 6
    • 6
    • 5
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 378-395
    Reaktivität
    • 173
    • 105
    • 55
    • 14
    • 10
    • 9
    • 7
    • 6
    • 6
    • 4
    • 4
    • 1
    • 1
    • 1
    • 1
    Maus
    Wirt
    • 161
    • 32
    • 6
    • 1
    Ratte
    Klonalität
    • 154
    • 45
    Monoklonal
    Konjugat
    • 103
    • 10
    • 10
    • 8
    • 7
    • 6
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    Dieser HSF1 Antikörper ist konjugiert mit PerCP
    Applikation
    • 159
    • 102
    • 78
    • 52
    • 41
    • 36
    • 28
    • 28
    • 27
    • 24
    • 8
    • 5
    • 4
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF), Gel Shift (GS), Immunocytochemistry (ICC)
    Spezifität
    Detects ~85 kDa (unstressed cell lysates), and~95 kDa (heat shocked cell lysates).
    Kreuzreaktivität
    Rind (Kuh), Meerschweinchen, Hamster, Human, Affe, Maus, Kaninchen, Ratte
    Aufreinigung
    Protein G Purified
    Immunogen
    Purified recombinant mouse HSF1 protein, with epitope mapping to amino acids 378-395
    Klon
    10H8
    Isotyp
    IgG1
    Top Product
    Discover our top product HSF1 Primärantikörper
  • Applikationshinweise
    • WB (1:1000)
    • IHC (1:1000)
    • ICC/IF (1:200)
    • optimal dilutions for assays should be determined by the user.
    Kommentare

    1 μg/ml of ABIN2484631 was sufficient for detection of HSF1 in 20 μg of heat shocked HeLa cell lysate by ECL immunoblot analysis using Goat anti-rat IgG: HRP as the secondary antibody

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    1 mg/mL
    Buffer
    PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    4 °C
    Informationen zur Lagerung
    Conjugated antibodies should be stored at 4°C
  • Target
    HSF1 (Heat Shock Factor Protein 1 (HSF1))
    Andere Bezeichnung
    HSF1 (HSF1 Produkte)
    Synonyme
    hsf1 antikoerper, Hsf antikoerper, xhsf1 antikoerper, AA960185 antikoerper, HSTF1 antikoerper, HSF1 antikoerper, HSF antikoerper, HSF1c antikoerper, hsf1a antikoerper, hsf1b antikoerper, zHSF1a antikoerper, zHSF1b antikoerper, hstf1 antikoerper, heat shock factor antikoerper, Heat Shock Factor antikoerper, heat shock factor 1 antikoerper, heat shock transcription factor 1 antikoerper, heat shock transcription factor 1 S homeolog antikoerper, hsf1 antikoerper, hsf-1 antikoerper, Hsf1 antikoerper, HSF1 antikoerper, hsf1.S antikoerper
    Hintergrund
    HSF1, or heat shock factor 1, belongs to a family of Heat Shock transcription factors that activate the transcription of genes encoding products required for protein folding, processing, targeting, degradation, and function (2). The up-regulation of HSP (heat shock proteins) expression by stressors is achieved at the level of transcription through a heat shock element (HSE) and a transcription factor (HSF) (3, 4, 5). Most HSFs have highly conserved amino acid sequences. On all HSFs there is a DNA binding domain at the N-terminus. Hydrophobic repeats located adjacent to this binding domain are essential for the formation of active trimers. Towards the C-terminal region another short hydrophobic repeat exists, and is thought to be necessary for suppression of trimerization (6). There are two main heat shock factors, 1 and 2. Mouse HSF1 exists as two isoforms, however in higher eukaryotes HSF1 is found in a diffuse cytoplasmic and nuclear distribution in un-stressed cells. Once exposed to a multitude of stressors, it localizes to discrete nuclear granules within seconds. As it recovers from stress, HSF1 dissipates from these granules to a diffuse nuceloplasmic distribution. HSF2 on the other hand is similar to mouse HSF1, as it exists as two isoforms, the alpha form being more transcriptionally active than the smaller beta form (7, 8). Various experiments have suggested that HFS2 may have roles in differentiation and development (9, 10, 11).
    Gen-ID
    15499
    NCBI Accession
    NP_032322
    UniProt
    P38532
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