DNMT1 Antikörper (AA 637-650) (HRP)

Produktnummer ABIN2486007

Produktdetails anti-DNMT1 Antikörper (HRP)

Target: DNMT1 (DNA (Cytosine-5)-Methyltransferase 1 (DNMT1))

Bindungsspezifität: AA 637-650

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser DNMT1 Antikörper ist konjugiert mit HRP

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Chromatin Immunoprecipitation (ChIP)

Spezifität: Detects ~180 kDa. It will cross-react with mouse DNMT1.

Kreuzreaktivität: Fisch, Human, Maus, Zebrafisch (Danio rerio)

Aufreinigung: Protein G Purified

Immunogen: Raised against a synthetic peptide corresponding to amino acids 637-650 of human DNMT1

Klon: 60B1220-1

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise:

• WB (1:1000)
• IHC (1:1000)
• optimal dilutions for assays should be determined by the user.

Kommentare:

2 μg/ml of ABIN2486007 was sufficient for detection of Dnmt1 in 10 μg of mouse ES cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: PBS, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Conjugated antibodies should be stored at 4°C

Details zu DNMT1

Target: DNMT1 (DNA (Cytosine-5)-Methyltransferase 1 (DNMT1))

Andere Bezeichnung: DNMT1 (DNMT1 Produkte)

Hintergrund: Methylation of DNA at cytosine residues plays an important role in regulation of gene expression, genomic imprinting and is essential for mammalian development. Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic DNA may be linked with development of cancer. To date, 3 families of mammalian DNA methyltransferase genes have been identified which include Dnmt1, Dnmt2 and Dnmt3. Dnmt1 is constitutively expressed in proliferating cells and inactivation of this gene causes global demethylation of genomic DNA and embryonic lethality. Dnmt2 is expressed at low levels in adult tissues and its inactivation does not affect DNA methylation or maintenance of methylation. The Dnmt3 family members, Dnmt3a and Dnmt3b, are strongly expressed in ES cells but their expression is down regulated in differentiating ES cells and is low in adult somatic tissue. Dnmt1 co-purifies with the retinoblastoma (Rb) tumour suppressor gene product, E2F1, and HDAC1. Dnmt1 also cooperates with Rb to repress transcription from promoters containing E2F-binding sites suggesting a link between DNA methylation, histone deacetylase and sequence-specific DNA binding activity, as well as a growth-regulatory pathway that is disrupted in nearly all cancer cells (1-6).

Gen-ID: 1786

NCBI Accession: NP_001370

UniProt: P26358

Pathways: SARS-CoV-2 Protein Interaktom, Phosphorylierungen bei SARS-CoV-2 Infektion