SOD1 Antikörper (N-Term) (Atto 390)

Produktnummer ABIN2486233

Der Kaninchen Polyklonal Anti-SOD1-Antikörper wurde für WB, ELISA, ICC und IF validiert. Er ist geeignet, SOD1 in Proben von Human zu detektieren.

Kurzübersicht für SOD1 Antikörper (N-Term) (Atto 390) (ABIN2486233)

Target: SOD1 (Superoxide Dismutase 1, Soluble (SOD1))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SOD1 Antikörper ist konjugiert mit Atto 390

Applikation: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF)

Produktdetails anti-SOD1 Antikörper (Atto 390)

Bindungsspezifität: N-Term

Spezifität: Recognizes a conformation specific epitope where the beta barrel is unfolded.

Kreuzreaktivität: Human, Maus, Ratte

Aufreinigung: Protein A Purified

Immunogen: Within 50 AA of N-terminal region of SOD1, unfolded beta barrel region

Anwendungsinformationen

Applikationshinweise:

• WB (1:1000)
• ICC/IF (1:100)
• optimal dilutions for assays should be determined by the user.

Kommentare:

1μg/ml of ABIN2486233 was sufficient for detection of the unfolded beta barrel of SOD1 by colorimetric dot blot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: PBS, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Conjugated antibodies should be stored at 4°C

Details zu SOD1

Target: SOD1 (Superoxide Dismutase 1, Soluble (SOD1))

Andere Bezeichnung: SOD1

Hintergrund: Superoxide dismutase (SOD) is an endogenously produced intracellular enzyme present in almost every cell in the body (2). It works by catalyzing the dismutation of the superoxide radical O2ˉ to O2 and H2O2, which are then metabolized to H2O and O2 by catalase and glutathione peroxidase (1,4). In general, SODs play a major role in antioxidant defense mechanisms (3). There are two main types of SOD in mammalian cells. One form (SOD1) contains Cu and Zn ions as a homodimer and exists in the cytoplasm. The two subunits of 16 kDa each are linked by two cysteines forming an intra-subunit disulphide bridge (2). Misfolding of SOD1 has been implicated in Amyotrophic lateral sclerosis (ALS). Therefore conformation specific antibodies such as Anti-SOD1 (UbetaB), which targets an unfolded region of the beta barrel of SOD1, are useful for determining the conformation of SOD1 in affected tissues (5). This antibody can be used in conjunction with Anti-SOD1 (EDI) (SPC-206D) which detects an exposed dimer interface (EDI) of SOD1.

Gen-ID: 6647

UniProt: P00441

Pathways: Sensory Perception of Sound, Transition Metal Ion Homeostasis