Phosphothreonine Antikörper (Atto 488)

Produktnummer ABIN2486267

Produktdetails anti-Phosphothreonine Antikörper (Atto 488)

Target: Phosphothreonine

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Phosphothreonine Antikörper ist konjugiert mit Atto 488

Applikation: Western Blotting (WB), Immunoprecipitation (IP), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF)

Spezifität: Detects proteins phosphorylated on threonine residues. Does not cross-react with phosphotyrosine.

Aufreinigung: Peptide Affinity Purified

Immunogen: Phosphothreonine conjugated to KLH

Anwendungsinformationen

Applikationshinweise:

• WB (1:500)
• ICC/IF (1:60)
• ELISA (1:2000)
• IP (1:100)
• optimal dilutions for assays should be determined by the user.

Kommentare:

2 μg/ml of ABIN2486267 was sufficient for detection of phosphorylation signal in western blot analysis using mouse spleen extract treated with Vanadium.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.25 mg/mL

Buffer: PBS, 50 % glycerol, 0.01 % sodium azide, Storage buffer may change when conjugated

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Conjugated antibodies should be stored at 4°C

Details zu Phosphothreonine

Target: Phosphothreonine

Abstract: Phosphothreonine Produkte

Substanzklasse: Amino Acid

Hintergrund: Protein phosphorylation is an important posttranslational modification that serves many key functions to regulate a protein's activity, localization, and protein-protein interactions. Phosphorylation is catalyzed by various specific protein kinases, which involves removing a phosphate group from ATP and covalently attaching it to to a recipient protein that acts as a substrate. Most kinases act on both serine and threonine, others act on tyrosine, and a number (dual specificity kinases) act on all three. Because phosphorylation can occur at multiple sites on any given protein, it can therefore change the function or localization of that protein at any time (1). Changing the function of these proteins has been linked to a number of diseases, including cancer, diabetes, heart disease, inflammation and neurological disorders (2-4).