CRYAB Antikörper (PE)

Produktnummer ABIN2486814

Produktdetails anti-CRYAB Antikörper (PE)

Target: CRYAB (Crystallin, alpha B (CRYAB))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CRYAB Antikörper ist konjugiert mit PE

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Spezifität: Detects ~22 kDa. Does not cross-react with αA-crystallin.

Kreuzreaktivität: Huhn, Rind (Kuh), Human, Maus, Ratte

Aufreinigung: Affinity Purified

Immunogen: Synthetic peptide corresponding to human alpha B crystallin conjugated to KLH

Anwendungsinformationen

Applikationshinweise:

• WB (1:5000)
• ICC/IF (1:120)
• optimal dilutions for assays should be determined by the user.

Kommentare:

A 1:5000 dilution of ABIN2486814 was sufficient for detection of alpha B crystallin in 20 μg of HeLa cell lysate by ECL immunoblot analysis.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Conjugated antibodies should be stored at 4°C

Details zu CRYAB

Target: CRYAB (Crystallin, alpha B (CRYAB))

Andere Bezeichnung: Alpha B Crystallin (CRYAB Produkte)

Synonyme: CMD1II antikoerper, CRYA2 antikoerper, CTPP2 antikoerper, CTRCT16 antikoerper, HSPB5 antikoerper, MFM2 antikoerper, Crya-2 antikoerper, Crya2 antikoerper, HspB5 antikoerper, AACRYA antikoerper, CRYAB antikoerper, cryab antikoerper, cryab2 antikoerper, wu:fe37f08 antikoerper, zgc:91937 antikoerper, crystallin alpha B antikoerper, crystallin, alpha B antikoerper, hypothetical protein antikoerper, crystallin, alpha B, a antikoerper, crystallin, alpha B, b antikoerper, CRYAB antikoerper, Cryab antikoerper, ZK1128.7 antikoerper, cryaba antikoerper, cryabb antikoerper

Hintergrund: The alpha-crystallins are major water-soluble lens structural proteins of the vertebrate eye that are related to the small heat shock protein family. The alpha-crystallins possess structural and functional similarities with HSP25 and HSP27 (1). Mammalian lens cystallins are divided into alpha, beta and gamma families. Alpha and beta families are further divided into acidic and basic groups (Alpha-A and Alpha-B respectively). In the lens, alpha-crystallin primarily functions to maintain proper refractive index, however it can also function as a molecular chaperone that binds to the denatured proteins, keeping them in solution and thereby maintaining the translucency of the lens. When cellular stress occurs, alpha-crystallin enters its' phosphorylated state and may serve a structural control function and play a role in protein maintenance (2). In addition to their interaction with proteins, alpha-crystallins also interact with native molecules such as membrane proteins, Golgi matrix protein, structural proteins, nuclear proteins and DNA (3, 4, 5, 6, and 7). Two other functions are an autokinase activity and participation in the intracellular architecture, and it has also been proven that both alpha-A and B prevent apoptosis by inhibiting caspases (8). Specifically, alpha-B cystallin is found in many cells and organs outside the lens, and alpha B is overexpressed in several neurological disorders and in cell lines under stress conditions (9).

Gen-ID: 1410

NCBI Accession: NP_001876

UniProt: P02511