This antibody recognizes the (Mr 150-170 kDa) cell surface glycoprotein expressed in a pan-myeloid fashion. This antibody also reacts with osteoclasts in giant cell tumors of bone (osteoclastoma), clear cell chondrosarcoma and aneurysmal bone cysts.
Kreuzreaktivität (Details)
Species reactivity (tested):Human.
Produktmerkmale
Synonyms: Aminopeptidase N, ANPEP, APN, PEPN, Alanyl aminopeptidase, Microsomalaminopeptidase, Aminopeptidase M, gp150, Myeloid plasma membrane glycoprotein CD13
Aufreinigung
Protein G Chromatography.
Immunogen
A cell suspension containing osteoclasts from osteoclastomas
Suitable for use in Flow Cytometry and Immunohistochemistry (acetone-fixed frozensections). We recommend using 1 μg to stain 1.0 x 10^6 cells in Flow Cytometric applications. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Konzentration
0.1 mg/mL (OD280nm)
Buffer
0.01 M PBS, pH 7.2 containing 2 mM EDTA, 1 % BSA as stabilizer and 0.09 % Sodium Azide as preservative.
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung
4 °C
Informationen zur Lagerung
Store the antibody undiluted at 2-8 °C. DO NOT FREEZE! Shelf life: one year from despatch.
CD13 also known as Aminopeptidase N is a zinc dependent exopeptidase which cleaves amino acids from the N-termini of target proteins including neuropeptides and cytokines. CD13 is expressed on all cells of the myeloid lineage as well as epithelial cells of the intestine, placenta, liver and kidney. CD13 is upregulated in a number of tumour types including lung, melanoma, thyroid and hepatocarcinoma and has been shown to correlate with poor prognosis. CD13 is a crucial molecule in tumour angiogenesis. CD13 is also the receptor for viruses from the coronavirus family.Synonyms: ANPEP, APN, Alanyl aminopeptidase, Aminopeptidase M, Aminopeptidase N, Microsomal aminopeptidase, Myeloid plasma membrane glycoprotein CD13, PEPN, gp150