Bovine Coronavirus Antikörper

Produktnummer ABIN263581

Produktdetails anti-Bovine Coronavirus Antikörper

Target: Bovine Coronavirus (BCoV)

Reaktivität: Bovine Coronavirus (BCoV)

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Bovine Coronavirus Antikörper ist unkonjugiert

Applikation: Enzyme Immunoassay (EIA)

Produktmerkmale: Synonyms: Spike glycoprotein, S glycoprotein

Aufreinigung: Protein G chromatography

Reinheit: > 95 % pure (SDS-PAGE)

Immunogen: Bovine corona virus. Hybridization of Sp2/0 myeloma cells with spleen cells from Balb/cmice.

Klon: 5A4

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: ELISA. HIT (hemagglutination inhibition titer).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.8mg/mL (OD280nm E0.1%=1.4)

Buffer: PBS, pH 7.4 containing 0.09 % Sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C. DO NOT FREEZE!
Shelf life: one year from despatch.

Haltbarkeit: 12 months

Details zu Bovine Coronavirus

Target: Bovine Coronavirus (BCoV)

Abstract: BCoV Produkte

Substanzklasse: Virus

Hintergrund: Bovine Corona Virus Spike glycoprotein is cleaved into S1 and S2 Spike proteins. The precursor is processed into S1 and S2 by host cell furin or furin-like protease to yield the mature S1 and S2 proteins. The cleavage site between S1 and S2 requires the optimal sequence [KR]-X-[KR]-R.S1 attaches the virion to the cell membrane by binding to 9-O-acetylated sialic acid containing proteins, initiating the infection.S2 is a class I viral fusion protein. Under the current model, the protein has at least 3 conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes.Synonyms: E2 protein, Peplomer protein, S glycoprotein, Spike glycoprotein

UniProt: P25193