This antibody Detects a ~70 kDa protein corresponding to the Molecular Mass of inducible HSP70 on SDS PAGE Immunoblots. The mapped epitope is in the region of amino acid residues 436-503. Does not cross-react with HSC70 (HSP73).
ELISA (10). Western blot (7,8,14)1 μg/mL was sufficient for detection of HSP70 in 20 μg of HeLa lysate by colorimetric
Beschränkungen
Nur für Forschungszwecke einsetzbar
Konzentration
1.0 mg/mL
Buffer
PBS, pH 7.2, 0.09 % Sodium Azide, 50 % Glycerol
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handhabung
Avoid repeated freezing and thawing.
Lagerung
4 °C/-20 °C
Informationen zur Lagerung
Store the antibody at 2-8 °C for one month or (in aliquots) at -28 °C for longer. Shelf life: one year from despatch.
Haltbarkeit
12 months
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APG-2 antikoerper, HS24/P52 antikoerper, HSPH2 antikoerper, RY antikoerper, hsp70 antikoerper, hsp70RY antikoerper, CG31354 antikoerper, HSP70 antikoerper, Hsp70Bb antikoerper, hsp70B antikoerper, hsp70Bb-prime antikoerper, DmelCG5834 antikoerper, CG5834 antikoerper, HSPA1 antikoerper, HSP70B' antikoerper, HSPA6 antikoerper, ARABIDOPSIS HEAT SHOCK PROTEIN 70 antikoerper, ATHSP70 antikoerper, heat shock protein 70 antikoerper, LOC100305036 antikoerper, hsc70 antikoerper, Hsp70 antikoerper, Hsp70-1 antikoerper, Hsp70.1 antikoerper, hsp68 antikoerper, Hsp110 antikoerper, irp94 antikoerper, HSP70-2 antikoerper, HSPA1B antikoerper, HSPA2 antikoerper, hsp70-5 antikoerper, HSP70-1 antikoerper, HSP70.1 antikoerper, HSP70.2 antikoerper, heat shock protein family A (Hsp70) member 4 antikoerper, CG5834 gene product from transcript CG5834-RA antikoerper, heat shock protein 70 antikoerper, heat shock protein family A (Hsp70) member 6 antikoerper, heat shock 70kDa protein 2 antikoerper, heat shock 70 kD protein cognate antikoerper, Hsp70 family chaperone antikoerper, Heat shock protein 70 antikoerper, Heat shock protein 70, putative antikoerper, heat shock protein 1B antikoerper, heat shock protein family A member 4 antikoerper, heat shock 70kDa protein 1A antikoerper, heat shock protein 1 antikoerper, Heat Shock Protein antikoerper, heat shock cognate 70-kd protein antikoerper, Heat shock 70 kDa protein 1A antikoerper, HSPA4 antikoerper, Hsp70Bbb antikoerper, HSP70 antikoerper, HSPA6 antikoerper, HSPA2 antikoerper, PCC7424_2419 antikoerper, Isop_1041 antikoerper, CGB_C3390W antikoerper, Bacsa_1698 antikoerper, dnaK-B antikoerper, LOC100305036 antikoerper, Hspa1b antikoerper, Hspa4 antikoerper, HSPA1A antikoerper, hsp1 antikoerper, hsp-70 antikoerper, hsp70 antikoerper, LOC108348108 antikoerper
Hintergrund
Hsp70 genes encode abundant heat-inducible 70- kDa hsps (hsp70s). In most eukaryotes hsp70 genes exist as part of a multigene family. They are found in most cellular compartments of eukaryotes including nuclei, mitochondria, chloroplasts, the endoplasmic reticulum and the cytosol, as well as in bacteria. The genes show a high degree of conservation, having at least 5O% identity (2). The N-terminal two thirds of hsp70s are more conserved than the C-terminal third. Hsp70 binds ATP with high affinity and possesses a weak ATPase activity which can be stimulated by binding to unfolded proteins and synthetic peptides (3). When hsc70 (constitutively expressed) present in mammalian cells was truncated, ATP binding activity was found to reside in an N-terminal fragment of 44 kDa which lacked peptide binding capacity. Polypeptide binding ability therefore resided within the Cterminal half (4). The structure of this ATP binding domain displays multiple features of nucleotide binding proteins (5). All hsp70s, regardless of location, bind proteins, particularly unfolded ones. The molecular chaperones of the hsp70 family recognize and bind to nascent polypeptide chains as well as partially folded intermediates of proteins preventing their aggregation and misfolding. The binding of ATP triggers a critical conformational change leading to the release of the bound substrate protein (6). The universal ability of hsp70s to undergo cycles of binding to and release from hydrophobic stretches of partially unfolded proteins determines their role in a great variety of vital intracellular functions such as protein synthesis, protein folding and oligomerization and protein transport.Synonyms: HSP70-1/HSP70-2, HSP70.1, HSPA1, HSPA1A, HSPA1B, Heat shock 70 kDa protein 1A/1B