HLA-DR Antikörper (Biotin)
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- Target Alle HLA-DR Produkte
- HLA-DR
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Reaktivität
- Human, Affe
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Wirt
- Maus
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Klonalität
- Monoklonal
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Konjugat
- Dieser HLA-DR Antikörper ist konjugiert mit Biotin
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Applikation
- Flow Cytometry (FACS)
- Produktmerkmale
- Synonyms: HLA-DR, HLA class II histocompatibility antigen DR, MHC class II antigen DR
- Klon
- 423L
- Isotyp
- IgG2a
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- Applikationshinweise
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Flow Cytometry. Monitoring of B cells in peripheral blood. Analysis of B subsets. Study of B cell activation. Study of B cell neoplasms.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Protokoll
- PBMC: Add 10 ul of MAB/10e6 PBMC in 100 ul PBS. Mix gently and incubate for 15 minutes at 2-8°C. Wash twice with PBS and analyze or fix with 0. 5% v/v of paraformaldehyde in PBS andanalyze. WHOLE BLOOD: Add 10 ul of MAB/100 ul of whole blood. Mix gently and incubate for 15 minutes at room temperature (20°C). Lyse the whole blood. Wash once with PBS and analyze or fix with 0. 5% v/v of paraformaldehyde in PBS andanalyze. See instrument manufacturers instructions for Lysed Whole Blood andImmunofluorescence analysis with a flow cytometer or microscope.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Buffer
- PBS containing 0.2 % protein carrier and 0.08 % Sodium Azide as preservative.
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Lagerung
- 4 °C
- Informationen zur Lagerung
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Store the antibody undiluted at 2-8 °C. Do Not Freeze!
Shelf life: One year from despatch. - Haltbarkeit
- 12 months
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- Target
- HLA-DR
- Andere Bezeichnung
- HLA Class II DR (HLA-DR Produkte)
- Hintergrund
- Identification of human B cell and T cell subsets associated with approximately 10 % of peripheral blood lymphocytes 28-34,000 M.W. surface antigen, also low density on monocytes and macrophages.Synonyms: HLA class II histocompatibility antigen DR, HLA-DR, MHC class II antigen DR
- Pathways
- Human Leukocyte Antigen (HLA) in Adaptive Immune Response
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