Carboxy Methyl Lysine Antikörper (methylated)

Produktnummer ABIN264528

Produktdetails anti-Carboxy Methyl Lysine Antikörper

Target: Carboxy Methyl Lysine (CML)

Bindungsspezifität: methylated

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser Carboxy Methyl Lysine Antikörper ist unkonjugiert

Applikation: Enzyme Immunoassay (EIA), Western Blotting (WB)

Aufreinigung: CML-protein Sepharose affinity chromatography

Immunogen: Carboxymethylated Keyhole Limpet Hemocyanin (CML-KLH). Prepared by the reaction of the protein with glyoxylic acid.

Anwendungsinformationen

Applikationshinweise: Western Blot. ELISA: 1: 4,000 - 1: 20,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1,0 mg/mL (OD280nm, E0.1% = 1.35)

Buffer: 75 mM PBS, pH 7.2 containing 75 mM Sodium chloride and 0.5 mM EDTA and 0.02 % Sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu Carboxy Methyl Lysine

Target: Carboxy Methyl Lysine (CML)

Andere Bezeichnung: Carboxy Methyl Lysine (CML Produkte)

Synonyme: ALL antikoerper, BCR1 antikoerper, CML antikoerper, D22S11 antikoerper, D22S662 antikoerper, PHL antikoerper, BCR, RhoGEF and GTPase activating protein antikoerper, BCR antikoerper

Substanzklasse: Amino Acid

Hintergrund: Carboxymethyl Lysine is formed by the non enzymatic Schiff base reaction of glucose with proteins, followed by an Amadori rearrangement and oxidation that leaves only a carboxymethyl group attached to the lysine. The levels of CML adducts accumulate over time and have been used as an indicator of both serum glucose levels and oxidative protein damage. Elevated serum CML modified proteins have been associated with diabetes and may contribute to diabetic retinopathy, nephropathy and angiopathy.Synonyms: CML, Carboxymethyl-lysine