Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
Kreuzreaktivität (Details)
Histone-H-1 100%, No reaction with Histone-H-2b, Histone-H-3 or Histone-H-4Very low crossreaction with Histone-H-core
Aufreinigung
purified
Immunogen
Histone-1 antibody was raised in sheep using Intact calf histone H1 complexed with RNA. as the immunogen.
Applikationshinweise
IF: 1:100, IP: 1:20, WB: 1:300 Optimal conditions should be determined by the investigator.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Konzentration
Lot specific
Buffer
Protein G purified and supplied in PBS, pH 7.4 .
Handhabung
Avoid repeated freeze/thaw cycles. Dilute only prior to immediate use.
Lagerung
-20 °C/-80 °C
Informationen zur Lagerung
Aliquot and store at -20 °C for short term storage, -70 °C for long-term storage.
Target
Histone 1
Hintergrund
Histones are highly alkaline proteins found in eukaryotic cell nuclei, which package and order the DNA into structural units called nucleosomes. They are the chief protein components of chromatin, acting as spools around which DNA winds, and play a role in gene regulation.