In sandwich ELISA, no cross-reactivity with mouse or rat IL-33.
Aufreinigung
The antibody was purified by antigen affinity chromatography, and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.
Immunogen
AA 112-270 (Accession# NM_033439) was expressed in E. coli.
IL-33, also known as NFHEV and DVS 27, is a 30 kD proinflammatory protein that belongs to IL-1 superfamily. IL-33 is constitutively expressed in smooth muscle and airway epithelia. It is upregulated in arterial smooth muscle, dermal fibroblasts, and keratinocytes following IL-1α or IL-1β stimulation. IL-33 interacts with IL1RL1/ST2 receptor and IL-1 receptor accessory protein (IL1RAP) in order to activate intracellular molecules in the NF-κB and MAP kinase signaling pathways that drive production of type 2 cytokines (e.g. IL-5 and IL-13) from polarized Th2 cells. Recombinant IL-33 has been shown to enhance Th2-associated immune responses and potently increase mast cell proliferation and cytokine production.