Reaktivität: Human
WB, ELISA
Wirt: Kaninchen
Polyclonal
unconjugated
Applikationshinweise
Optimal working dilution should be determined by the investigator.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Konzentration
0.5 mg/mL
Buffer
phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide. Final antibody concentration is 0.5 mg/mL.
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung
4 °C
Informationen zur Lagerung
Upon receipt, store between 2°C and 8°C.
Target
Nuclear Factor kappa B p50 (NFkB p50)
Andere Bezeichnung
NF-B p50
Synonyme
NF-KB1 antikoerper, NF-kappaB antikoerper, NF-kappaB1 antikoerper, p105 antikoerper, p50 antikoerper, p50/p105 antikoerper, nuclear factor of kappa light polypeptide gene enhancer in B cells 1, p105 antikoerper, Nfkb1 antikoerper
Hintergrund
NF-κB/p50 (nuclear factor kappa light chain enhancer in B cells p50, NF-κB1) is a member of the Rel/dorsal family. This ubiquitously expressed nuclear protein is one subunit of the NF-kappa B complex consisting of a 65 kD transactivating subunit and a 50 kD DNA binding subunit. Both subunits are derived from larger precursor proteins. NF-κB acts as a transcriptional activator and was originally identified as an activator of kappa light chain in B cells. NF-κB/p50 is bound (with p65) to IκB inhibitor in cytoplasm in inactive form. Phosphorylation of IκB releases and NF- κB which goes to the nucleus to activate gene expression. NF-κB can be activated with LPS, TNF-α, phorbol ester, or IL-1. The 4D1 monoclonal antibody reacts with human NF-κB p50 and has been shown to be useful for Western blotting.