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5-Methylcytosine Antikörper

WB, MeDIP, FACS, IF, DB, ICC, IHC, ELISA Wirt: Maus Monoclonal 33D3 unconjugated
Produktnummer ABIN2668982
  • Target
    5-Methylcytosine
    Reaktivität
    Bitte anfragen
    Wirt
    • 22
    • 8
    • 3
    Maus
    Klonalität
    • 24
    • 9
    Monoklonal
    Konjugat
    • 16
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Unkonjugiert
    Applikation
    • 19
    • 15
    • 13
    • 9
    • 8
    • 7
    • 7
    • 5
    • 5
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Methylated DNA Immunoprecipitation (MeDIP), Flow Cytometry (FACS), Immunofluorescence (IF), Dot Blot (DB), Immunocytochemistry (ICC), Immunohistochemistry (IHC), ELISA
    Aufreinigung
    Protein A Chromatography
    Immunogen
    Clone 33D3 recognizes the modified base 5-methylcytidine found in plant and vertebrate DNA.
    Klon
    33D3
    Isotyp
    IgG
  • Applikationshinweise
    Optimal working dilution should be determined by the investigator.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Konzentration
    1 μg/μL
    Buffer
    Purified IgG in 10 mM phosphate, 0.15 M NaCl and 0.01 % thimerosal. Thimerosal is highly toxic.
    Handhabung

    Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

    Lagerung
    -20 °C
    Informationen zur Lagerung
    Antibodies in solution can be stored at -20 °C for 2 years.
    Haltbarkeit
    6 months
  • Borghesan, Fusilli, Rappa, Panebianco, Rizzo, Oben, Mazzoccoli, Faulkes, Pata, Agodi, Rezaee, Minogue, Warren, Peterson, Sedivy, Douet, Buschbeck, Cappello, Mazza, Pazienza, Vinciguerra: "DNA Hypomethylation and Histone Variant macroH2A1 Synergistically Attenuate Chemotherapy-Induced Senescence to Promote Hepatocellular Carcinoma Progression." in: Cancer research, Vol. 76, Issue 3, pp. 594-606, (2016) (PubMed).

    McPherson, Fullston, Kang, Sandeman, Corbett, Owens, Lane: "Paternal under-nutrition programs metabolic syndrome in offspring which can be reversed by antioxidant/vitamin food fortification in fathers." in: Scientific reports, Vol. 6, pp. 27010, (2016) (PubMed).

    Banh, Iorio, Marcotte, Xu, Cojocari, Rahman, Pawling, Zhang, Sinha, Rose, Isasa, Zhang, Wu, Virtanen, Hitomi, Habu, Sidhu, Koizumi, Wilkins, Kislinger, Gygi, Schofield, Dennis, Wouters, Neel: "PTP1B controls non-mitochondrial oxygen consumption by regulating RNF213 to promote tumour survival during hypoxia." in: Nature cell biology, Vol. 18, Issue 7, pp. 803-813, (2016) (PubMed).

    Harrison, Cornett, Goldfarb, DaRosa, Li, Yan, Dickson, Guo, Cantu, Kaustov, Brown, Arrowsmith, Erie, Major, Klevit, Krajewski, Kuhlman, Strahl, Rothbart: "Hemi-methylated DNA regulates DNA methylation inheritance through allosteric activation of H3 ubiquitylation by UHRF1." in: eLife, Vol. 5, (2016) (PubMed).

    Yang, Ma, Li, Zhang, Wan, Du, Li, Yang, Lu: "Iodine-125 irradiation inhibits invasion of gastric cancer cells by reactivating microRNA-181c expression." in: Oncology letters, Vol. 12, Issue 4, pp. 2789-2795, (2016) (PubMed).

    Ivanov, Kals, Kacevska, Barragan, Kasuga, Rane, Metspalu, Milani, Ingelman-Sundberg: "Ontogeny, distribution and potential roles of 5-hydroxymethylcytosine in human liver function." in: Genome biology, Vol. 14, Issue 8, pp. R83, (2014) (PubMed).

    Xu, Wang, Long, Zhang, Wei, Xing, Gu, Xie: "Stress-mediated p38 activation promotes somatic cell reprogramming." in: Cell research, Vol. 23, Issue 1, pp. 131-41, (2013) (PubMed).

    Colquitt, Allen, Barnea, Lomvardas: "Alteration of genic 5-hydroxymethylcytosine patterning in olfactory neurons correlates with changes in gene expression and cell identity." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 110, Issue 36, pp. 14682-7, (2013) (PubMed).

    Bocker, Tuorto, Raddatz, Musch, Yang, Xu, Lyko, Breiling: "Hydroxylation of 5-methylcytosine by TET2 maintains the active state of the mammalian HOXA cluster." in: Nature communications, Vol. 3, pp. 818, (2012) (PubMed).

    Habib, Fares, Bourgeois, Bella, Bernardino, Hernandez-Blazquez, de Capoa, Niveleau: "DNA global hypomethylation in EBV-transformed interphase nuclei." in: Experimental cell research, Vol. 249, Issue 1, pp. 46-53, (1999) (PubMed).

  • Target
    5-Methylcytosine
    Substanzklasse
    Chemical
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