Dieser Angiotensin I Converting Enzyme 1 Antikörper ist unkonjugiert
Applikation
Flow Cytometry (FACS)
Marke
BD Pharmingen™
Produktmerkmale
The BB9 Monoclonal antibody specifically binds Angiotensin-converting enzyme (ACE) which is also known as Dipeptidyl carboxypeptidase 1 (DCP1) or CD143. CD143 is a type I transmembrane glycoprotein that functions as a zinc metallopeptidase involved with the metabolism of angiotensin, bradykinin, and other oligopeptides. It is expressed on primitive hematopoietic progenitor cells (HPC). BB9 reacts with stromal cells, a minor subset of human bone marrow cells, it does not react with peripheral blood leucocytes. BB9 recognizes a subset of CD34+CD90+ cells that demonstrate low or absent expression of CD38, low retention of Rhodamine 123 and are Ki-67-negative. CD34+BB9+ cells are able to sustain hematopoiesis in pre-CFU culture stimulated with IL-3, IL-6, G-CSF, and SCF. BB9 also reacts with a subset of CD34+ cells from mobilized peripheral blood. This antibody was generated using human bone marrow-derived stromal cells as immunogen. Flow cytometric analysis of CD143 expression on human bone marrow cells. Human bone marrow cells were stained with APC Anti-Human CD34 (hematopoietic progenitor cell marker) and Purified Mouse Anti-Human Angiotensin Converting Enzyme (CD143) followed by FITC Goat Anti-Mouse IgG/IgM (Cat. No. 555988). The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometric analysis of CD143 expression on human bone marrow cell line. HEL 92.1.7 (Human bone marrow erythroblast, ATCC TIB-180) cells were stained with either Purified Mouse IgG1, κ Isotype Control (Cat. No. 555746, Dashed line histogram) or Purified Mouse Anti-Human Angiotensin Converting Enzyme (CD143) (Solid line historgram), followed by FITC Goat Anti-Mouse IgG/IgM (Cat. No. 555988). The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable cells.
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung
4 °C
Informationen zur Lagerung
Store undiluted at 4°C.
Sinka, Biasch, Khazaal, Péault, Tavian: "Angiotensin-converting enzyme (CD143) specifies emerging lympho-hematopoietic progenitors in the human embryo." in: Blood, Vol. 119, Issue 16, pp. 3712-23, (2012) (PubMed).
Zambidis, Park, Yu, Tam, Levine, Yuan, Pryzhkova, Péault: "Expression of angiotensin-converting enzyme (CD143) identifies and regulates primitive hemangioblasts derived from human pluripotent stem cells." in: Blood, Vol. 112, Issue 9, pp. 3601-14, (2008) (PubMed).
Jokubaitis, Sinka, Driessen, Whitty, Haylock, Bertoncello, Smith, Péault, Tavian, Simmons: "Angiotensin-converting enzyme (CD143) marks hematopoietic stem cells in human embryonic, fetal, and adult hematopoietic tissues." in: Blood, Vol. 111, Issue 8, pp. 4055-63, (2008) (PubMed).
Target
Angiotensin I Converting Enzyme 1 (ACE)
(Angiotensin I Converting Enzyme (Peptidyl-Dipeptidase A) 1 (ACE))