The GAME-46 antibody reacts with the common β2 chain of LFA-1 (CD11a/CD18, αLβ2 integrin), Mac-1 (CD11b/CD18, αMβ2 integrin), and gp150, 95 (CD11c/CD18, αXβ2 integrin). Expression of CD18 is limited to leukocytes, where it is widely distributed in consort with each of the three integrin α chains (CD11a, CD11b, and CD11c). Among splenocytes, NK cells have the highest density of CD18, and T lymphocytes express a higher density than the remaining cells. The β2 integrins are important mediators of leukocyte-endothelium interactions. It has been reported that GAME-46 antibody blocks in vitro adhesion of LFA-1-expressing cells to the ligands ICAM-1, ICAM-2 and ICAM-3 and of Mac-1-expressing cells to ICAM-1, C3bi, and fibrinogen.
BD Pharmingen™ Purified Rat Anti-Mouse CD18 - Purified - Clone GAME-46 - Isotype Rat IgG1, κ - Reactivity Ms - 0.1 mg
Aufreinigung
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen
LFA-1 (CD11a/CD18) from mouse T-cell hybridoma TAM8C41
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung
4 °C
Informationen zur Lagerung
Store undiluted at 4°C.
Driessens, van Hulten, Zuurbier, La Rivière, Roos: "Inhibition and stimulation of LFA-1 and Mac-1 functions by antibodies against murine CD18. Evidence that the LFA-1 binding sites for ICAM-1, -2, and -3 are distinct." in: Journal of leukocyte biology, Vol. 60, Issue 6, pp. 758-65, (1997) (PubMed).
Springer: "Traffic signals for lymphocyte recirculation and leukocyte emigration: the multistep paradigm." in: Cell, Vol. 76, Issue 2, pp. 301-14, (1994) (PubMed).
Springer: "Adhesion receptors of the immune system." in: Nature, Vol. 346, Issue 6283, pp. 425-34, (1990) (PubMed).