The EH12.1 monoclonal antibody specifically binds to CD279. CD279 is an immunoregulatory receptor that is expressed on activated T cells, B cells and myeloid cells and contains an immunoreceptor tyrosine-based inhibitory motif (ITIM) in the cytoplasmic region. Mice deficient in CD279 show a breakdown of peripheral tolerance and manifest multiple autoimmune symptoms. PD-L1 and PD-L2 are ligands of CD279 and are members of the B7 gene family. Interaction of CD279:PD-Ligands results in inhibition of T cell proliferation and cytokine secretion. Reports suggest that the B7/CTLA-4 pathway functions primarily to attenuate, limit, and/or terminate naive T-cell activation in secondary lymphoid organs. The PD-ligand:CD279 pathway, on the other hand, may primarily attenuate, limit, and/or terminate T-, B-, and myeloid cell activation/effector function at sites of inflammation in the periphery.
BD Pharmingen™ Purified NA/LE Mouse anti-Human CD279 (PD-1) - NA/LE - Clone EH12.1 (also known as EH12) - Isotype Mouse IgG1, κ - Reactivity Hu - 0.5 mg
Aufreinigung
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Sterilität
Sterile filtered,0.2 μm filtered
Endotoxin-Niveau
Endotoxin level is ≤0.01 EU/μg (≤0.001 ng/μg) of protein as determined by the LAL assay.
Optimal working dilution should be determined by the investigator.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Konzentration
1.0 mg/mL
Buffer
No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.
Konservierungsmittel
Without preservative
Handhabung
This preparation contains no preservatives, thus it should be handled under aseptic conditions.
Lagerung
4 °C
Informationen zur Lagerung
Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.
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