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- Target Alle CD4 Antikörper anzeigen
- CD4
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Reaktivität
- Ratte
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Wirt
- Maus
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Klonalität
- Monoklonal
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Konjugat
- Dieser CD4 Antikörper ist konjugiert mit Biotin
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Applikation
- Flow Cytometry (FACS)
- Marke
- BD Pharmingen™
- Produktmerkmale
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The OX-35 clone has been reported to react with the CD4 antigen on most thymocytes, a subpopulation of mature T lymphocytes (i.e. MHC class II-restricted T cells, including most T helper cells), monocytes, macrophages, some dendritic cells, and microglia. CD4 is an antigen coreceptor on the T-cell surface that interacts with MHC class II molecules on antigen-presenting cells. It participates in T-cell activation through it's association with the T-cell receptor complex and protein tyrosine kinase lck. The OX-35 clone has been reported to bind to a different epitope of CD4 than that recognized by the W3/25 and OX-38 clones. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature. Two-color analysis of the expression of CD4 on rat splenic leukocytes. Lewis splenocytes were simultaneously stained with biotinylated anti-rat CD4 mAb clone OX-35 (right panel) and PE conjugated anti-rat CD3 mAb clone G4.18 (Cat. No. 554833), followed by avidin-FITC (Cat. No. 554057). The CD3-negative CD4-dim cells are the monocyte/macrophage population. Flow cytometry was performed on a BD FACScan™ flow cytometry system.
BD Pharmingen™ Biotin Mouse Anti-Rat CD4 - Biotin - Clone OX-35 - Isotype Mouse IgG2a, κ - Reactivity Rat - 0.5 mg - Aufreinigung
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- Immunogen
- Rat T-cell blasts
- Klon
- OX
- Isotyp
- IgG2a kappa
- Top Product
- Discover our top product CD4 Primärantikörper
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- Applikationshinweise
- Optimal working dilution should be determined by the investigator.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Konzentration
- 0.5 mg/mL
- Buffer
- Aqueous buffered solution containing ≤0.09 % sodium azide.
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handhabung
- The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.
- Lagerung
- 4 °C
- Informationen zur Lagerung
- Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
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Dendritic cell heterogeneity in vivo: two functionally different dendritic cell populations in rat intestinal lymph can be distinguished by CD4 expression." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 161, Issue 3, pp. 1146-55, (1998) (PubMed).
: "Immunohistochemical study of amoeboid microglial cells in fetal rat brain." in: Journal of anatomy, Vol. 189 ( Pt 3), pp. 567-74, (1997) (PubMed).
: "Tissue digestion with dispase substantially reduces lymphocyte and macrophage cell-surface antigen expression." in: Journal of immunological methods, Vol. 194, Issue 1, pp. 71-5, (1996) (PubMed).
: "Cell-surface marker analysis of rat thymic dendritic cells." in: Immunology, Vol. 79, Issue 2, pp. 298-304, (1993) (PubMed).
: "The T cell receptor as a multicomponent signalling machine: CD4/CD8 coreceptors and CD45 in T cell activation." in: Annual review of immunology, Vol. 10, pp. 645-74, (1992) (PubMed).
: "The biologic roles of CD2, CD4, and CD8 in T-cell activation." in: Annual review of immunology, Vol. 7, pp. 579-99, (1989) (PubMed).
: "Authentic T helper CD4 (W3/25) antigen on rat peritoneal macrophages." in: The Journal of experimental medicine, Vol. 162, Issue 1, pp. 117-27, (1985) (PubMed).
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Dendritic cell heterogeneity in vivo: two functionally different dendritic cell populations in rat intestinal lymph can be distinguished by CD4 expression." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 161, Issue 3, pp. 1146-55, (1998) (PubMed).
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- Target
- CD4
- Andere Bezeichnung
- CD4 (CD4 Produkte)
- Synonyme
- cd4 antikoerper, L3T4 antikoerper, Ly-4 antikoerper, CD4mut antikoerper, W3/25 antikoerper, p55 antikoerper, CD4 molecule antikoerper, carcinoembryonic antigen-related cell adhesion molecule 5 antikoerper, T-cell surface glycoprotein CD4 antikoerper, CD4 antigen antikoerper, Cd4 molecule antikoerper, CD4 antikoerper, LOC100125525 antikoerper, cd4 antikoerper, Cd4 antikoerper
- Pathways
- T-Zell Rezeptor Signalweg, Maintenance of Protein Location, CXCR4-mediated Signaling Events
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