Dieser CD45RA Antikörper ist konjugiert mit Biotin
Applikation
Flow Cytometry (FACS)
Marke
BD Pharmingen™
Produktmerkmale
The 14.8 clone has been reported to react with an exon A-dependent epitope of the CD45 protein, which is found at high density on B cells and at low density on peripheral T cytotoxic/suppressor cells and a very small subset of thymocytes. Nearly all B-lineage cells, including B-cell precursors in fetal liver and adult bone marrow and Ig-secreting cells, but not hematopoietic stem cells or myeloid progenitors, have been reported to be detectable by mAb 14.8. CD45 is a member of the Protein Tyrosine Phosphatase (PTP) family: Its intracellular (COOH-terminal) region contains two PTP catalytic domains, and the extracellular region is highly variable due to alternative splicing of exons 4, 5, and 6 (designated A, B, and C, respectively), plus, differing levels of glycosylation. The CD45 isoforms detected in the mouse are cell type-, maturation-, and activation state-specific. The CD45 isoforms play complex roles in T-cell and B-cell antigen receptor signal transduction. mAb 14.8 has been reported to enhance the proliferative effect of PHA on purified spleen T cells, possibly by replacing a signal normally delivered by accessory cells, to enhance isotype switching during in vitro B-cell responses, and to inhibit antigen-induced p21 [ras] activation. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature.
BD Pharmingen™ Biotin Rat Anti-Mouse CD45RA - Biotin - Clone 14.8 - Isotype Rat IgG2b, κ - Reactivity Ms - 0.1 mg
Aufreinigung
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung
The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.
Lagerung
4 °C
Informationen zur Lagerung
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
George, Rath, Shroff, Wang, Durdik: "Ligation of CD45 on B cells can facilitate production of secondary Ig isotypes." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 152, Issue 3, pp. 1014-21, (1994) (PubMed).
Goff, Huby: "Characterization of constitutive and strain-dependent subsets of CD45RA+ cells in the thymus." in: International immunology, Vol. 4, Issue 11, pp. 1303-11, (1993) (PubMed).
Inoue, Asano, Matsuoka, Furutani-Seiki, Aizawa, Nishimura, Shirai, Tada: "Distinction of mouse CD8+ suppressor effector T cell clones from cytotoxic T cell clones by cytokine production and CD45 isoforms." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 150, Issue 6, pp. 2121-8, (1993) (PubMed).
Hathcock, Laszlo, Dickler, Sharrow, Johnson, Trowbridge, Hodes: "Expression of variable exon A-, B-, and C-specific CD45 determinants on peripheral and thymic T cell populations." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 148, Issue 1, pp. 19-28, (1992) (PubMed).
Rogers, Pilapil, Hayakawa, Romain, Parker: "CD45 alternative exon expression in murine and human CD4+ T cell subsets." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 148, Issue 12, pp. 4054-65, (1992) (PubMed).
Marvel, Poirier, Lightstone: "Anti-CD45RA antibodies increase the proliferation of mouse T cells to phytohemagglutinin through the interleukin 2/interleukin 2 receptor pathway." in: European journal of immunology, Vol. 19, Issue 11, pp. 2005-10, (1990) (PubMed).
Kincade, Lee, Watanabe, Sun, Scheid: "Antigens displayed on murine B lymphocyte precursors." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 127, Issue 6, pp. 2262-8, (1982) (PubMed).
Kincade: "Formation of B lymphocytes in fetal and adult life." in: Advances in immunology, Vol. 31, pp. 177-245, (1982) (PubMed).