Reacts with CD59, a 19 kDa glycosylphosphatidylinositol (GPI)-anchored glycoprotein, expressed on hematopoietic and non-hematopoietic cells. Because of its role in interaction with complement activated products, CD59 has been termed membrane-attack-complex-inhibitory factor (MACIF), homologus restriction factor (HRF20), membrane inhibitor of reactive lysis (MIRL) and protectin. It inhibits the cytolytic activity of complement by binding to C8 and C9, thereby blocking the assembly of the membrane attack complex. CD59 also participates in spontaneous T-cell/erythrocyte adhesion, interacts with CD2 and plays a role in T-cell activation. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature. Profile of glycosylphosphatidylinositol (GPI) anchor-defective mutant cell line analyzed on a FACScan Profile of K562 cells expressing glycosylphosphatidylinositol (GPI) anchor protein analyzed on a FACScan
CD59
Reaktivität: Human
FACS
Wirt: Maus
Monoclonal
MEM-43
FITC
Applikationshinweise
Optimal working dilution should be determined by the investigator.
Probenmenge
20 μL
Beschränkungen
Nur für Forschungszwecke einsetzbar
Buffer
Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung
The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.
Lagerung
4 °C
Informationen zur Lagerung
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Davies, Lachmann: "Membrane defence against complement lysis: the structure and biological properties of CD59." in: Immunologic research, Vol. 12, Issue 3, pp. 258-75, (1994) (PubMed).
Deckert, Kubar, Bernard: "CD58 and CD59 molecules exhibit potentializing effects in T cell adhesion and activation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 148, Issue 3, pp. 672-7, (1992) (PubMed).
Deckert, Kubar, Zoccola, Bernard-Pomier, Angelisova, Horejsi, Bernard: "CD59 molecule: a second ligand for CD2 in T cell adhesion." in: European journal of immunology, Vol. 22, Issue 11, pp. 2943-7, (1992) (PubMed).
Guesdon, Ternynck, Avrameas: "The use of avidin-biotin interaction in immunoenzymatic techniques." in: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 27, Issue 8, pp. 1131-9, (1979) (PubMed).