CD8 Antikörper
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- Target Alle CD8 Antikörper anzeigen
- CD8
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Reaktivität
- Human, Cynomolgus, Rhesusaffen, Pavian
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Wirt
- Maus
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Klonalität
- Monoklonal
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Konjugat
- Dieser CD8 Antikörper ist unkonjugiert
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Applikation
- Flow Cytometry (FACS)
- Marke
- BD Pharmingen™
- Produktmerkmale
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The RPA-T8 monoclonal antibody specifically binds to CD8 alpha (CD8α). CD8α is a type I transmembrane glycoprotein and a member of the immunoglobulin superfamily. CD8α is expressed by the majority of thymocytes, by subpopulations of αβ T cells and γδ T cells and by some NK cells. Cell surface CD8α is expressed either as a disulfide-linked homodimer (CD8αα) or as a heterodimer (CD8αβ) when disulfide-bonded to a CD8 beta chain (CD8β). CD8-positive αβ T cells coexpress both CD8αα homodimers and CD8αβ heterodimers whereas some γδ T cells and NK cells express CD8αα homodimers. CD8 plays important roles in T cell activation and selection. The extracellular IgSF domain of CD8α binds to a non-polymorphic determinant on HLA class I molecules (α3 domain) and enables CD8 to function as a co-receptor with MHC class I-restricted TCR during T cell recognition of antigen. The cytoplasmic domain of CD8α associates with Lck, a Src family protein tyrosine kinase that is involved in intracellular signaling. The RPA-T8 and HIT8a monoclonal antibodies are not cross-blocking. This clone has been reported to react with a subset of peripheral blood lymphocytes, but not monocytes nor granuloyctes, of baboon and both rhesus and cynomolgus macaque monkey. In general, a higher frequency of CD8+ and CD4+CD8+ lymphocytes are observed in non-human primates compared to normal human donors. Profile of anti-CD8 reactivity on peripheral blood lymphocytes of rhesus macaque (macaca mulatta) analyzed by flow cytometry.
BD Pharmingen™ Purified Mouse Anti-Human CD8 - Purified - Clone RPA-T8 - Isotype Mouse IgG1, κ - Reactivity Hu, Bab, Cyno, Rhe - 0.1 mg - Aufreinigung
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- Klon
- RPA
- Isotyp
- IgG1 kappa
- Top Product
- Discover our top product CD8 Primärantikörper
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- Applikationshinweise
- Optimal working dilution should be determined by the investigator.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Konzentration
- 0.5 mg/mL
- Buffer
- Aqueous buffered solution containing ≤0.09 % sodium azide.
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Lagerung
- 4 °C
- Informationen zur Lagerung
- Store undiluted at 4°C.
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Lymphocyte subsets and expression of differentiation markers in blood and lymphoid organs of rhesus monkeys." in: Cytometry, Vol. 29, Issue 4, pp. 351-62, (1998) (PubMed).
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Lymphocyte subsets and expression of differentiation markers in blood and lymphoid organs of rhesus monkeys." in: Cytometry, Vol. 29, Issue 4, pp. 351-62, (1998) (PubMed).
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- Target
- CD8
- Andere Bezeichnung
- CD8 (CD8 Produkte)
- Synonyme
- CD8 antikoerper, Leu2 antikoerper, MAL antikoerper, p32 antikoerper, CD8a molecule antikoerper, CD8A antikoerper
- Hintergrund
- Synonyms: CD8α, CD8A, CD8 alpha, Leu2, MAL, T8, p32
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