Western Blotting (WB), ELISA, Neutralization (Neut)
Marke
BD Pharmingen™
Produktmerkmale
The BVD4-1D11 antibody reacts with mouse interleukin-4 (IL-4). The immunogen used to generate the BVD4-1D11 hybridoma was recombinant mouse IL-4. This is a neutralizing antibody. This antibody is routinely tested by intracellular staining and sandwich ELISA. Other applications were tested during antibody development only or reported in the literature. Expression of IL-4 by MiCK-2 positive control cells. MiCK-2 positive control cells (Cat. No. 554653) were stained with 0.125 μg of FITC-conjugated rat anti-mouse IL-4 antibody by using the BD Pharmingen staining protocol (see left panel). To demonstrate specificity of staining, the binding of FITC-BVD4-1D11 was blocked by the preincubation of the conjugated antibody with recombinant mouse IL-4 (0.25 μg, Cat. No. 550067, see middle panel), and by preincubation of the fixed/permeabilized cells with unlabeled BVD4-1D11 antibody (5 μg, Cat. No. 554386, see right panel). The quadrant markers for the bivariate dot plots were set based on the autofluorescence control, and verified with the recombinant cytokine blocking and unlabeled antibody blocking specificity controls.
BD Pharmingen™ Purified Rat Anti-Mouse IL-4 - Purified - Clone BVD4-1D11 - Isotype Rat IgG2b - Reactivity Ms - 0.1 mg
Aufreinigung
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung
4 °C
Informationen zur Lagerung
Store undiluted at 4°C.
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