The MECA-89 antibody reacts with mucosal vascular addressin MAdCAM-1. In the fetus and neonate, MAdCAM-1 is the predominant vascular addressin on the high endothelial venules (HEV) of peripheral lymph nodes. In adult mice, MAdCAM-1 is preferentially expressed in mucosal lymphoid tissues and lamina propria, it is also expressed on sinus-lining cells in the spleen. MAdCAM-1 expression is upregulated on the HEV of peripheral lymph nodes in adult NOD mice and is involved in the development of diabetes and insulitis. Furthermore, there is evidence that IFN-γ can induce MAdCAM-1 expression in non-mucosal sites in adult mice. MAdCAM-1 is a predominant ligand for integrin α4β7, a lymphocyte mucosal homing receptor, and a facultative ligand for CD62L (L-selectin). MECA-89 mAb binds to the second domain of MAdCAM-1 and does not block MAdCAM-1-dependent binding in vitro. Source of the immunogen was endothelial cells from BALB/c mouse mesenteric and peripheral lymph nodes. Immunohistochemical staining of mouse MadCAM-1: Frozen sections of normal mouse small intestine were reacted with the anti-MadCAM-1 antibody. Cells of the lamina propria and the mucosal lymphoid tissue expressing MadCAM-1 can be identified by the brown labeling of their cell membranes. Amplification 20X.
BD Pharmingen™ Purified Rat Anti-Mouse MAdCAM-1 - Purified - Clone MECA-89 - Isotype Rat IgG2a, κ - Reactivity Ms - 1 mL
Aufreinigung
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.