H2-T23 Antikörper (AA 161-179) (Biotin)

Produktnummer ABIN2689887

Produktdetails anti-H2-T23 Antikörper (Biotin)

Target: H2-T23 (Histocompatibility 2, T Region Locus 23 (H2-T23))

Bindungsspezifität: AA 161-179

Reaktivität: Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser H2-T23 Antikörper ist konjugiert mit Biotin

Applikation: Flow Cytometry (FACS)

Marke: BD Pharmingen™

Produktmerkmale: The 6A8.6F10.1A6 monoclonal antibody reacts with the Qa-1[b] alloantigen, which is a nonclassical MHC class I (Class Ib) molecule encoded by the T23 gene of the H-2 complex. Qa-1 associates with β2-microglobulin and is expressed at low levels on most leukocytes and many other cell types. Its level of cell-surface expression is upregulated by IFNγ or specific peptides. Qa-1 is an oligomorphic molecule which presents a limited pool of peptides to T lymphocytes bearing αβ and γδTCR and binds to a large subpopulation of NK cells. In fact, Qa-1[b] is the ligand for CD94/NKG2A, CD94/NKG2C, and CD94/NKG2E receptors, which are expressed on NK cells. Furthermore, it has been reported that Qa-1 expressed on activated B lymphocytes is involved in immunoregulation by inducing T-cell-mediated suppression of antibody responses. The 6A8.6F10.1A6 mAb can detect Qa-1[b] on activated splenocytes from C57BL/6 and BALB/c mice (both Qa-1[b]), but not from A/j mice (Qa-1[a]), and it can block target cell recognition by CTLs specific for Qa-1-presented antigen.

BD Pharmingen™ Biotin Mouse Anti-Mouse Qa-1[b] - Biotin - Clone 6A8.6F10.1A6 - Isotype Mouse IgG1, κ - Reactivity Ms - 0.5 mg

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Qa-1[b] aa. 161-179 Peptide

Klon: 6A8-6F10-1A6

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.

Lagerung: 4 °C

Informationen zur Lagerung: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Referenzen für anti-H2-T23 Antikörper (Biotin) (ABIN2689887)

Vance, Jamieson, Raulet: "Recognition of the class Ib molecule Qa-1(b) by putative activating receptors CD94/NKG2C and CD94/NKG2E on mouse natural killer cells." in: The Journal of experimental medicine, Vol. 190, Issue 12, pp. 1801-12, (2000) (PubMed).

Lo, Ong, Metcalf, Soloski: "T cell responses to Gram-negative intracellular bacterial pathogens: a role for CD8+ T cells in immunity to Salmonella infection and the involvement of MHC class Ib molecules." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 162, Issue 9, pp. 5398-406, (1999) (PubMed).

Salcedo, Bousso, Ljunggren, Kourilsky, Abastado: "The Qa-1b molecule binds to a large subpopulation of murine NK cells." in: European journal of immunology, Vol. 28, Issue 12, pp. 4356-61, (1999) (PubMed).

Sivakumar, Gunturi, Salcedo, Schatzle, Lai, Kurepa, Pitcher, Seaman, Lemonnier, Bennett, Forman, Kumar: "Cutting edge: expression of functional CD94/NKG2A inhibitory receptors on fetal NK1.1+Ly-49- cells: a possible mechanism of tolerance during NK cell development." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 162, Issue 12, pp. 6976-80, (1999) (PubMed).

Vance, Kraft, Altman, Jensen, Raulet: "Mouse CD94/NKG2A is a natural killer cell receptor for the nonclassical major histocompatibility complex (MHC) class I molecule Qa-1(b)." in: The Journal of experimental medicine, Vol. 188, Issue 10, pp. 1841-8, (1999) (PubMed).

Noble, Zhao, Cantor: "Suppression of immune responses by CD8 cells. II. Qa-1 on activated B cells stimulates CD8 cell suppression of T helper 2 responses." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 160, Issue 2, pp. 566-71, (1998) (PubMed).

Soloski, DeCloux, Aldrich, Forman: "Structural and functional characteristics of the class IB molecule, Qa-1." in: Immunological reviews, Vol. 147, pp. 67-89, (1996) (PubMed).

Details zu H2-T23

Target: H2-T23 (Histocompatibility 2, T Region Locus 23 (H2-T23))

Andere Bezeichnung: Qa-1[b] (H2-T23 Produkte)

Synonyme: 37b antikoerper, 37c antikoerper, H-2T23 antikoerper, H2-Qa1 antikoerper, Qa-1 antikoerper, Qa-1(b) antikoerper, Qa1 antikoerper, Qed-1 antikoerper, T18c antikoerper, T18c(37) antikoerper, T23b antikoerper, T23d antikoerper, histocompatibility 2, T region locus 23 antikoerper, H2-T23 antikoerper

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process