R-Phycoerythrin Antikörper (Biotin)

Produktnummer ABIN2689889

Produktdetails anti-R-Phycoerythrin Antikörper (Biotin)

Target: R-Phycoerythrin (Phycoerythrin, R-)

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser R-Phycoerythrin Antikörper ist konjugiert mit Biotin

Applikation: Flow Cytometry (FACS)

Marke: BD Pharmingen™

Produktmerkmale: The E31-1459 antibody reacts with R-Phycoerythrin (PE), a commonly used fluorochrome for flow cytometry, alone or as a tandem conjugate with other fluorochromes (eg, PE-Cy™7). The binding of the E31-1459 antibody to PE does not quench the fluorescence of the PE molecule. The use of biotinylated E31-1459 in a three-step staining procedure with Streptavidin-PE and a PE-conjugated primary antibody can specifically amplify the PE fluorescence. Amplification of PE-conjugated anti-mouse CD19 staining of BALB/c mouse splenocytes. Mouse splenocytes were incubated with PE-conjugated anti-mouse CD19 mAb 1D3 (Cat. No. 557399/553786) alone or followed by biotinylated mAb E31- 1459 and PE-conjugated Streptavidin (Cat. No. 554061, right panel). The overlayed histograms correspond to stained splenocytes (shaded histogram), unstained splenocytes (left panel, unshaded histogram), or splenocytes stained with biotinylated mAb E31-1459 and PE-conjugated Streptavidin (right panel, unshaded histogram). The signal-to-noise ratio of median fluorescence intensity (MFI) of the CD19+ population to the CD19- population in both panels (shaded histograms) is 87 for Panel A and 172 for right panel, demonstrating an approximate two-fold specific amplification of staining. Data shown is for gated lymphocytes based on light scatter. Flow cytometry was performed on a BD FACSCalibur™ flow cytometry system.

BD Pharmingen™ Biotin Mouse Anti-R-Phycoerythrin - Biotin - Clone E31-1459 - Isotype Mouse IgG1, κ - 0.1 mg

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: R-phycoerythrin

Klon: E31

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Handhabung: The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.

Lagerung: 4 °C

Informationen zur Lagerung: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Details zu R-Phycoerythrin

Target: R-Phycoerythrin (Phycoerythrin, R-)

Andere Bezeichnung: R-Phycoerythrin (R-Phycoerythrin Produkte)

Substanzklasse: Chemical