Actin Antikörper (pan)

Produktnummer ABIN271986

Produktdetails anti-Actin Antikörper

Target: Actin

Bindungsspezifität: pan

Reaktivität: Human, Ratte, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Actin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Spezifität: This antibody detects endogenous levels ofActin-pan (α/β/γ) protein (region surrounding Gln49)

Kreuzreaktivität (Details): Species reactivity (expected):Mouse and RatSpecies reactivity (tested):Human.

Aufreinigung: Affinity-chromatography

Reinheit: > 95 % (by SDS-PAGE)

Anwendungsinformationen

Applikationshinweise: ELISA: 1: 10000approx. 1: 20000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200. IF: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1,0 mg/mL

Buffer: PBS, pH 7.2, 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu Actin

Target: Actin

Abstract: Actin Produkte

Synonyme: Act57 antikoerper, Act57A antikoerper, Act57b antikoerper, Actin antikoerper, BEST:RE38067 antikoerper, C antikoerper, CG10067 antikoerper, CG30294 antikoerper, Dmel\\CG10067 antikoerper, M antikoerper, T10 antikoerper, act 57A antikoerper, act57A antikoerper, act57B antikoerper, act57b antikoerper, actin antikoerper, anon-EST:CL2c12 antikoerper, 143060_f_at antikoerper, CG7478 antikoerper, D antikoerper, Dmel\\CG7478 antikoerper, act 79B antikoerper, act79B antikoerper, mus79B antikoerper, Act(88F) antikoerper, Act88-F antikoerper, Act88f antikoerper, CG5178 antikoerper, Dmel\\CG5178 antikoerper, E antikoerper, Ifm(3)7 antikoerper, act 88F antikoerper, act88F antikoerper, rsd antikoerper, Act87e antikoerper, CG18290 antikoerper, Dmel\\CG18290 antikoerper, act 87E antikoerper, act87E antikoerper, mus87E antikoerper, Act88F antikoerper, Dsim\\GD19025 antikoerper, GD19025 antikoerper, dsim_GLEANR_2829 antikoerper, 42A antikoerper, A antikoerper, AFFX-Dros-ACTIN_M_r_at antikoerper, Act antikoerper, Act42 antikoerper, Act42a antikoerper, Actin/BAP47 antikoerper, BAP47 antikoerper, CG12051 antikoerper, Dmel\\CG12051 antikoerper, act 42A antikoerper, act42A antikoerper, cytoplasmic antikoerper, Actin 57B antikoerper, Actin 79B antikoerper, Actin 88F antikoerper, Actin 87E antikoerper, Actin-like protein C08B11.6 antikoerper, hypothetical protein antikoerper, ACT_COLSC Actin (ISS) antikoerper, Actin 42A antikoerper, actin gamma 1 antikoerper, actin antikoerper, Actin, gamma 1 antikoerper, si:ch211-241j12.3 antikoerper, Act57B antikoerper, Act79B antikoerper, Act88F antikoerper, Act87E antikoerper, arp-6 antikoerper, Dsim\Act88F antikoerper, ACTIN antikoerper, Act42A antikoerper, ACTG1 antikoerper, LOC548170 antikoerper, Bm1_16810 antikoerper, IDA5 antikoerper, LOC100342017 antikoerper, si:ch211-241j12.3 antikoerper

Hintergrund: Beta actin is one of six different actin isoforms which have been identified. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Be aware when using beta actin antibodies as loading controls that although beta actin is normally the best protein to use as a loading control, under some conditions its amounts can vary between samples. In particular, expression of actin in adipose tissue is very low (Spiegelman and Farmer, Cell, 1982, 29(1):53-60) "in cells undergoing adipose differentiation actin synthesis decreases by 90 % ".

Molekulargewicht: approx. 45 kDa