ELISA: 1: 21000approx. 1: 5000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200. Other applications not tested. Optimal dilutions are dependent on conditions and should bedetermined by the user.
PBK (PDZ binding kinase) belongs to the serine/threonine protein kinase family. It is related to the dual specific mitogen-activated protein kinase kinase (MAPKK) family and phosphorylates MAP kinase p38. It is active only in mitosis. When phosphorylated it forms a complex with TP53, leading to TP53 destabilization and attenuation of G2/M checkpoint during doxorubicin-induced DNA damage. It makes a kinase-substrate complex with cdk1/cyclin B1 and PRC1 on microtubules during mitosis, enhances the cdk1/cyclin B1-dependent phosphorylation of PRC1 and thereby strongly promotes cytokinesis. It may play a role in the activation of lymphoid cells.Synonyms: CT84, Cancer/testis antigen 84, Lymphokine-activated killer T-cell-originated protein kinase, MAPKK-like protein kinase, Nori-3, PDZ-binding kinase, SPK, T-LAK cell-originated protein kinase