DCP1A Antikörper

Produktnummer ABIN272254

Produktdetails anti-DCP1A Antikörper

Target: DCP1A (DCP1 Decapping Enzyme Homolog A (S. Cerevisiae) (DCP1A))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser DCP1A Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Spezifität: This antibody detects endogenous levels of SMIF protein. (region surrounding Ser133)

Kreuzreaktivität (Details): Species reactivity (tested):Human, Mouse.

Aufreinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Reinheit: > 95 % pure by SDS-PAGE

Anwendungsinformationen

Applikationshinweise: ELISA: 1: 20000approx. 1: 40000. WB: 1: 500approx. 1: 1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: DO NOT FREEZE!

Lagerung: 4 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C.

Details zu DCP1A

Target: DCP1A (DCP1 Decapping Enzyme Homolog A (S. Cerevisiae) (DCP1A))

Andere Bezeichnung: DCP1A (DCP1A Produkte)

Synonyme: DCP1A antikoerper, 1110066A22Rik antikoerper, 4930568L04Rik antikoerper, AU019772 antikoerper, D14Ertd817e antikoerper, Mitc1 antikoerper, SMIF antikoerper, RGD1561298 antikoerper, mitc1 antikoerper, HSA275986 antikoerper, Nbla00360 antikoerper, SMAD4IP1 antikoerper, smad4ip1 antikoerper, smif antikoerper, decapping mRNA 1A antikoerper, decapping mRNA 1A L homeolog antikoerper, DCP1A antikoerper, Dcp1a antikoerper, dcp1a antikoerper, dcp1a.L antikoerper

Hintergrund: Signal transduction of TGF-beta superfamily members is regulated by Smad proteins. In particular, Smads influence specific gene transcription by relaying signals from the cell membrane to the nucleus. Smad4 plays an essential role in TGF-beta-induced transcriptional activation wherein phosphorylated receptor-associated Smads associate with Smad4. Furthermore, SMIF (Smad4-intereacting protein) and Smad4 complex with TGF-beta and BMP4. An increase in Smad4 concentration increases the translocation of this complex to the nucleus. SMIF and Smad4 interact directly through a EVH1/WH1 domain on SMIF and a proline-rich activation domain on Smad4. Smad4 is essential to nuclear translocation of SMIF as deletion of the Smad4-interacting domain (located in the N-terminal 100 amino acids) of SMIF eliminates TGF-β-induced nuclear translocation of SMIF (1). The human SMIF gene is ubiquitously expressed and encodes a protein with a relative molecular mass of 70 kDa.Synonyms: SMIF, Smad4-interacting transcriptional co-activator, Transcription factor SMIF, mRNA-decapping enzyme 1A

Molekulargewicht: approx. 75 kDa

Gen-ID: 55802

NCBI Accession: NP_060873

UniProt: Q9NPI6