APAF1 Antikörper (N-Term)

Produktnummer ABIN2745510

Produktdetails anti-APAF1 Antikörper

Target: APAF1 (Apoptotic Peptidase Activating Factor 1 (APAF1))

Bindungsspezifität: AA 1-97, N-Term

Reaktivität: Human, Maus

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser APAF1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunocytochemistry (ICC)

Spezifität: Recognizes the CARD domain of human and mouse Apaf-1.

Kreuzreaktivität: Human, Maus

Keine Kreuzreaktivität: Ratte (Rattus)

Kreuzreaktivität (Details): Does not cross-react with rat Apaf-1.

Reinheit: >95 % (SDS-PAGE)

Endotoxin-Niveau: <0.1EU/μg purified protein (LAL test, Lonza).

Immunogen: Recombinant mouse Apaf-1 (aa 1-97) containing the N-terminal CARD domain.

Klon: 18H2

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: In PBS containing 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short Term Storage: +4°C
Long Term Storage: -20°C
Stable for at least 1 year after receipt when stored at -20°C.

Haltbarkeit: 12 months

Referenzen für anti-APAF1 Antikörper (ABIN2745510)

Oladzad, Nikkhah, Hosseinkhani: "Optimization of Experimental Variables Influencing Apoptosome Biosensor in HEK293T Cells." in: Sensors (Basel, Switzerland), Vol. 20, Issue 6, (2020) (PubMed).

Tashakor, H-Dehkordi, OConnell, Gomez Ganau, Gozalbes, Eriksson, Hosseinkhani, Fearnhead: "A new split-luciferase complementation assay identifies pentachlorophenol as an inhibitor of apoptosome formation." in: FEBS open bio, Vol. 9, Issue 7, pp. 1194-1203, (2020) (PubMed).

Noori, Hosseini, Nikkhah, Hosseinkhani: "Apoptosome formation upon overexpression of native and truncated Apaf-1 in cell-free and cell-based systems." in: Archives of biochemistry and biophysics, Vol. 642, pp. 46-51, (2019) (PubMed).

Details zu APAF1

Target: APAF1 (Apoptotic Peptidase Activating Factor 1 (APAF1))

Andere Bezeichnung: Apaf-1 (APAF1 Produkte)

Hintergrund: Apaf-1 is a cytoplasmic protein that forms one of the central hubs in the apoptosis regulatory network. It contains (from the N terminal) a caspase recruitment domain (CARD), an ATPase domain (NB-ARC), few short helical domains and then several copies of the WD40 repeat domain. Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome, mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis.

UniProt: O88879

Pathways: p53 Signalweg, Apoptose, Caspase Kaskade in der Apoptose, Tube Formation, Positive Regulation of Endopeptidase Activity