ICAM-3/CD50 Antikörper

Produktnummer ABIN2749029

Produktdetails anti-ICAM-3/CD50 Antikörper

Target: ICAM-3/CD50 (ICAM3) (Intercellular Adhesion Molecule 3 (ICAM3))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser ICAM-3/CD50 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Functional Studies (Func)

Spezifität: The antibody MEM-04 reacts with an extracellular epitope of CD50 (ICAM-3), a 120-130 kDa type I membrane protein (immunoglobulin supergene family) expressed on leukocytes, endothelial cells and Langerhans cells, it is negative on platelets and erythrocytes. The MEM-04 blocks DC-SIGN (CD209) binding to ICAM-3 (epitope in D1).

Kreuzreaktivität (Details): Human

Aufreinigung: Purified by protein-A affinity chromatography.

Reinheit: > 95 % (by SDS-PAGE)

Endotoxin-Niveau: Endotoxin level is less than 0.01 EU/µg of the protein, as determined by the LAL test.

Immunogen: Leukocytes of a patient suffering from LGL-type leukemia.

Klon: MEM-04

Isotyp: IgG2b kappa

Anwendungsinformationen

Applikationshinweise: Functional application: The antibody MEM-04 blocks DC-SIGN (CD209) binding to ICAM-3 (epitope in D1).
Flow cytometry: Recommended dilution: 1-4 μg/mL.
Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/mL, positive tissue: tonsil.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4

Konservierungsmittel: Azide free

Lagerung: 4 °C

Informationen zur Lagerung: Store at 2-8°C. Do not freeze.

Details zu ICAM-3/CD50

Target: ICAM-3/CD50 (ICAM3) (Intercellular Adhesion Molecule 3 (ICAM3))

Andere Bezeichnung: CD50 (ICAM3 Produkte)

Synonyme: CD50 antikoerper, CDW50 antikoerper, ICAM-R antikoerper, ICAM-3 antikoerper, ICAM3 antikoerper, intercellular adhesion molecule 3 antikoerper, ICAM3 antikoerper, Icam3 antikoerper

Hintergrund: Intercellular adhesion molecule 3,CD50 (intracellular adhesion molecule 3, ICAM-3) is a transmembrane glycoprotein expressed by leukocytes, that serves as a counter-receptor for the lymphocyte function-associated antigen (LFA)-1 integrin. Besides functioning as an adhesive molecule that mediates e.g. the contact between T cells and antigen presenting cells, ICAM-3 regulates affinity of LFA-1 for ICAM-1 and induces T cell activation and proliferation. ICAM-3 plays an essential role in the initiation of the immune response both on T cells and antigen presenting cells and interacts also with CD209 (dendritic cell-specific ICAM-3-grabbing nonintegrin, DC-SIGN), a C-type lectin of dendritic cells and macrophages, this process is involved in dialogue between dendritic cells and granulocytes.,ICAM-3, ICAM-R

Gen-ID: 3385

UniProt: P32942