L-Selectin Antikörper

Produktnummer ABIN2749031

Produktdetails anti-L-Selectin Antikörper

Target: L-Selectin (SELL) (Selectin L (SELL))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser L-Selectin Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Functional Studies (Func)

Spezifität: The mouse monoclonal antibody DREG56 recognizes an extracellular epitope of CD62L / L-selectin, a 65-76 kDa cell surface protein, expressed by neutrophils, monocytes, and subsets of T, B, and NK cells, that interacts with specific carbohydrates exposed on activated endothelial cells.

Kreuzreaktivität (Details): Human

Aufreinigung: Purified by protein-A affinity chromatography.

Reinheit: > 95 % (by SDS-PAGE)

Endotoxin-Niveau: Endotoxin level is less than 0.01 EU/µg of the protein, as determined by the LAL test.

Immunogen: PMA-activated human peripheral blood leukocytes

Klon: DREG56

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Functional application: Particularly effective in blocking of lymphocyte CD62L-mediated binding to peripheral lymph node HEV.
Flow cytometry: Recommended dilution: 2 μg/mL.
Western blotting: Non-reducing conditions.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4

Konservierungsmittel: Azide free

Lagerung: 4 °C

Informationen zur Lagerung: Store at 2-8°C. Do not freeze.

Referenzen für anti-L-Selectin Antikörper (ABIN2749031)

Tu, Mao, Zheng, Liu, Chiu, Qin, Chan, Lam, Guan, Zhang, Guan, Yuen, Peiris, Lau: "Cytotoxic T lymphocytes established by seasonal human influenza cross-react against 2009 pandemic H1N1 influenza virus." in: Journal of virology, Vol. 84, Issue 13, pp. 6527-35, (2010) (PubMed).

Killock, Parsons, Zarrouk, Ameer-Beg, Ridley, Haskard, Zvelebil, Ivetic: "In Vitro and in Vivo Characterization of Molecular Interactions between Calmodulin, Ezrin/Radixin/Moesin, and L-selectin." in: The Journal of biological chemistry, Vol. 284, Issue 13, pp. 8833-45, (2009) (PubMed).

Xu, Chen, Shang, Cui, Luo, Chen, Ba, Zeng: "Critical role of Lck in L-selectin signaling induced by sulfatides engagement." in: Journal of leukocyte biology, Vol. 84, Issue 4, pp. 1192-201, (2008) (PubMed).

Jutila, Kurk, Jackiw, Knibbs, Stoolman: "L-selectin serves as an E-selectin ligand on cultured human T lymphoblasts." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 169, Issue 4, pp. 1768-73, (2002) (PubMed).

Kishimoto, Warnock, Jutila, Butcher, Lane, Anderson, Smith: "Antibodies against human neutrophil LECAM-1 (LAM-1/Leu-8/DREG-56 antigen) and endothelial cell ELAM-1 inhibit a common CD18-independent adhesion pathway in vitro." in: Blood, Vol. 78, Issue 3, pp. 805-11, (1991) (PubMed).

Kishimoto, Jutila, Butcher: "Identification of a human peripheral lymph node homing receptor: a rapidly down-regulated adhesion molecule." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 87, Issue 6, pp. 2244-8, (1990) (PubMed).

Details zu L-Selectin

Target: L-Selectin (SELL) (Selectin L (SELL))

Andere Bezeichnung: CD62L (SELL Produkte)

Synonyme: CD62L antikoerper, LAM1 antikoerper, LECAM1 antikoerper, LEU8 antikoerper, LNHR antikoerper, LSEL antikoerper, LYAM1 antikoerper, PLNHR antikoerper, TQ1 antikoerper, A.11 antikoerper, L-selectin antikoerper, LECAM-1 antikoerper, SELE antikoerper, AI528707 antikoerper, Lnhr antikoerper, Ly-22 antikoerper, Ly-m22 antikoerper, Lyam-1 antikoerper, Lyam1 antikoerper, selectin L antikoerper, selectin, lymphocyte antikoerper, SELL antikoerper, Sell antikoerper

Hintergrund: Selectin L,CD62L (L-selectin) is an adhesion glycoprotein that is constitutively expressed on the cell surface of leukocytes and mediates their homing to inflammatory sites and peripheral lymph nodes by enabling rolling along the venular wall. CD62L is also involved in activation-induced neutrophil aggregation. Activation-dependent CD62L shedding, however, counteracts neutrophil rolling. CD62L has also signaling roles including enhance of chemokine receptor expression. Similarly to CD62P, the major ligand of CD62L is PSGL-1 (P-selectin glycoprotein ligand-1).,L-selectin, LSEL, SELL, LAM-1, LECAM1, LEU8, LNHR, LYAM1, PLNHR

Gen-ID: 6402

UniProt: P14151