Transferrin Antikörper

Produktnummer ABIN2749040

Produktdetails anti-Transferrin Antikörper

Target: Transferrin (TF)

Reaktivität: Human, Schwein, Kaninchen

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Transferrin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Radioimmunoassay (RIA), Functional Studies (Func)

Spezifität: The antibody HTF-14 recognizes an epitope located in the N-terminal domain of human serum transferrin, a 77 kDa single polypeptide chain glycoprotein (member of the iron binding family of proteins). It is synthesised in the liver and consists of two domains each having a high affinity reversible binding site for Fe3+.

Keine Kreuzreaktivität: Rind (Kuh), Hund, Pferd, Schaf

Kreuzreaktivität (Details): Human, Rabbit, Porcine

Aufreinigung: Purified by protein-A affinity chromatography.

Reinheit: > 95 % (by SDS-PAGE)

Endotoxin-Niveau: Endotoxin level is less than 0.01 EU/µg of the protein, as determined by the LAL test.

Immunogen: Purified porcine transferrin.

Klon: HTF-14

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Functional application: The antibody HTF-14 blocks binding of transferrin to its receptor.
Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/mL, positive tissue: placenta.
Western blotting: non-reducing conditions, recommended dilution: 1-2 μg/mL.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4

Konservierungsmittel: Azide free

Lagerung: 4 °C

Informationen zur Lagerung: Store at 2-8°C. Do not freeze.

Referenzen für anti-Transferrin Antikörper (ABIN2749040)

Nováková, Dráberová, Schürmann, Czihak, Viklický, Dr-aber: "gamma-Tubulin redistribution in taxol-treated mitotic cells probed by monoclonal antibodies." in: Cell motility and the cytoskeleton, Vol. 33, Issue 1, pp. 38-51, (1996) (PubMed).

Bártek, Viklický, Stratil: "Phylogenetically more conservative epitopes among monoclonal antibody-defined antigenic sites of human transferrin are involved in receptor binding." in: British journal of haematology, Vol. 59, Issue 3, pp. 435-41, (1985) (PubMed).

Bártek, Viklický, Fran?k, Angelisová, Dráber, Jarosíková, N?mec, Verlová: "Monoclonal antibodies against transferrin. Precipitating mixtures and lack of inter-species cross-reactivity." in: Immunology letters, Vol. 4, Issue 5, pp. 231-5, (1982) (PubMed).

Details zu Transferrin

Target: Transferrin (TF)

Andere Bezeichnung: Transferrin (TF Produkte)

Synonyme: ltf antikoerper, pro1557 antikoerper, pro2086 antikoerper, mgc107777 antikoerper, LOC692564 antikoerper, TF antikoerper, LOC100144362 antikoerper, tf antikoerper, LTF antikoerper, TFEW antikoerper, conalbumin antikoerper, tf-b antikoerper, MGC64306 antikoerper, PRO1557 antikoerper, PRO2086 antikoerper, TFQTL1 antikoerper, AI266983 antikoerper, Cd176 antikoerper, HP antikoerper, Tf antikoerper, Tfn antikoerper, hpx antikoerper, Trf antikoerper, cb285 antikoerper, gavi antikoerper, id:ibd3238 antikoerper, id:ibd3525 antikoerper, sb:cb285 antikoerper, wu:fb57g06 antikoerper, wu:fb62h02 antikoerper, wu:fb63h10 antikoerper, wu:fb64h10 antikoerper, zgc:112154 antikoerper, 143958_at antikoerper, CG6186 antikoerper, Dmel\\CG6186 antikoerper, TSF1 antikoerper, anon-EST:Posey265 antikoerper, tsf1 antikoerper, Pro-TRH antikoerper, IL-5 antikoerper, STF I antikoerper, TRF1 antikoerper, sTF1 antikoerper, sTf antikoerper, tf1 antikoerper, transferrin antikoerper, serotransferrin antikoerper, transferrin (ovotransferrin) antikoerper, transferrin L homeolog antikoerper, melanotransferrin antikoerper, transferrin-a antikoerper, Transferrin 1 antikoerper, thyrotropin releasing hormone antikoerper, interleukin 5 antikoerper, TF antikoerper, LOC477072 antikoerper, tf antikoerper, Tf antikoerper, LOC100144362 antikoerper, tf.L antikoerper, LOC5575625 antikoerper, Trf antikoerper, tfa antikoerper, Tsf1 antikoerper, TRH antikoerper, IL5 antikoerper, LOC101085148 antikoerper, LOC100726872 antikoerper, trf antikoerper

Hintergrund: Transferrin,Transferrin is a monomeric glycoprotein of approximately 77 kDa, which serves as an iron-transporter. In normal plasma, transferrin has a concentration of 25-50 µmol / liter, and is usually about one-third saturated with iron, thus providing a large buffering capacity in case of an acute increase in plasma iron levels. Cells take up transferrin-iron complexes (holotransferrin) using transferrin receptor dimers. Upon binding of holotransferrin, the receptor is internalized by clathrin-mediated endocytosis. Acidification of endosomes by vesicular membrane proton pumps leads to dissociation of iron ions, whereas transferrin (apotransferrin) remains associated with its receptor (CD71) and recycles to the cell surface, where apotransferrin is released upon exposure to normal pH. Internalization of labeled transferrin thus represents an usefull approach to study endocytosis. Serum concentration rises in iron deficiency and pregnancy and falls in iron overload, infection and inflammatory conditions. Iron/transferrin complex is essential in haemoglobin synthesis and for certain types of cell division.,TFQTL1, PRO1557, PRO2086

Gen-ID: 7018

UniProt: P02787

Pathways: Transition Metal Ion Homeostasis