CD247 Antikörper (Tyr72)

Produktnummer ABIN2749060

Produktdetails anti-CD247 Antikörper

Target: CD247 (CD247 Molecule (CD247))

Bindungsspezifität: Tyr72

Reaktivität: Human, Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD247 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Spezifität: The mouse monoclonal antibody EM-26 recognizes phosphorylated intracellular tyrosine 72 of CD3 zeta chain (CD247), which is a component of TCR/CD3 complex expressed on T cells.

Kreuzreaktivität (Details): Human, Mouse

Aufreinigung: Purified by protein-A affinity chromatography.

Reinheit: > 95 % (by SDS-PAGE)

Endotoxin-Niveau: Low Endotoxin

Immunogen: A phospho specific peptide corresponding to the amino acids surrounding tyrosine 72 of mouse CD3 zeta linked to KLH

Klon: EM-26

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise: Western blotting: Recommended dilution: 2 - 5 μg/mL, positive control: Jurkat cells lysate treated with pervanadate, splenocyte lysate of Balb/c or F1 mouse treated with pervanadate, non-reducing conditions recommended.
Flow cytometry: Intracellular staining, recommended dilution: 1-9 μg/mL, positive control: Jurkat cells treated with pervanadate, T-cells from lymph nodes of OT-1 mouse treated with pervanadate.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store at 2-8°C. Do not freeze.

Referenzen für anti-CD247 Antikörper (ABIN2749060)

Dopfer, Schöpf, Louis-Dit-Sully, Dengler, Höhne, Klescová, Prouza, Suchanek, Reth, Schamel: "Analysis of novel phospho-ITAM specific antibodies in a S2 reconstitution system for TCR-CD3 signalling." in: Immunology letters, Vol. 130, Issue 1-2, pp. 43-50, (2010) (PubMed).

Details zu CD247

Target: CD247 (CD247 Molecule (CD247))

Andere Bezeichnung: CD3 zeta (CD247 Produkte)

Synonyme: CD3-ZETA antikoerper, CD3H antikoerper, CD3Q antikoerper, CD3Z antikoerper, T3Z antikoerper, TCRZ antikoerper, 4930549J05Rik antikoerper, A430104F18Rik antikoerper, AW552088 antikoerper, Cd3 antikoerper, Cd3-eta antikoerper, Cd3-zeta antikoerper, Cd3h antikoerper, Cd3z antikoerper, Cd3zeta antikoerper, T3z antikoerper, Tcrk antikoerper, Tcrz antikoerper, LOC100136514 antikoerper, LOC100136549 antikoerper, LOC100305023 antikoerper, CD247 antikoerper, TCRzeta antikoerper, CD247 molecule antikoerper, CD247 antigen antikoerper, CD3zeta-2 antikoerper, CD3zeta-1 antikoerper, Cd247 molecule antikoerper, CD247 antikoerper, Cd247 antikoerper, cd3z antikoerper, cd247 antikoerper

Hintergrund: CD247 Molecule,CD3 complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta (CD247). These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation.,CD3 zeta chain, CD247, T3Z, TCRz, IMD25

Gen-ID: 919

UniProt: P20963

Pathways: T-Zell Rezeptor Signalweg, CXCR4-mediated Signaling Events, Ubiquitin Proteasome Pathway