FOXP3 Antikörper (APC)

Produktnummer ABIN2749083

Produktdetails anti-FOXP3 Antikörper (APC)

Target: FOXP3 (Forkhead Box P3 (FOXP3))

Reaktivität: Human, Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser FOXP3 Antikörper ist konjugiert mit APC

Applikation: Flow Cytometry (FACS)

Spezifität: The mouse monoclonal antibody 3G3 recognizes N-terminal region of FoxP3, a 47-55 kDa transcription factor (intracellular antigen), which is the master regulator in the development and function of regulatory T cells.

Kreuzreaktivität (Details): Human, Mouse

Aufreinigung: Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Immunogen: Full-length His-tagged recombinant murine FoxP3

Klon: 3G3

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Flow cytometry: Recommended dilution: 1-2 μg/mL. Intracellular staining

Kommentare:

The purified antibody is conjugated with cross-linked Allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

Referenzen für anti-FOXP3 Antikörper (APC) (ABIN2749083)

Law, Hirschkorn, Owen, Biswas, Norris, Lanteri: "The importance of Foxp3 antibody and fixation/permeabilization buffer combinations in identifying CD4+CD25+Foxp3+ regulatory T cells." in: Cytometry. Part A : the journal of the International Society for Analytical Cytology, Vol. 75, Issue 12, pp. 1040-50, (2009) (PubMed).

Gavin, Torgerson, Houston, DeRoos, Ho, Stray-Pedersen, Ocheltree, Greenberg, Ochs, Rudensky: "Single-cell analysis of normal and FOXP3-mutant human T cells: FOXP3 expression without regulatory T cell development." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 103, Issue 17, pp. 6659-64, (2006) (PubMed).

Details zu FOXP3

Target: FOXP3 (Forkhead Box P3 (FOXP3))

Andere Bezeichnung: FoxP3 (FOXP3 Produkte)

Hintergrund: Forkhead box P3,FoxP3 (forkhead box protein 3), a highly conserved forkhead/winged-helix transcription factor, plays a crucial role in maintaining immune homeostasis by governing the development and function of regulatory T cells. It is constitutively expressed at high level in CD25+ CD4+ Treg cells and at low level in a CD25- CD4+ Treg cell subset. Defects in gene encoding FoxP3 protein cause the scurfy phenotype in mice, and in human the IPEX syndrome (immune dysfunction, polyendocrinopathy, enteropathy, X-linked syndrome), also known as X-linked autoimmunity-allergic dysregulation (XLAAD) syndrome.,Scurfin, JM2, AIID, IPEX, PIDX, XPID, DIETER

Gen-ID: 50943

UniProt: Q9BZS1

Pathways: Chromatin Binding, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation