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Fc gamma RII (CD32) Antikörper (PE)

CD32 Reaktivität: Human FACS Wirt: Maus Monoclonal 3D3 PE
Produktnummer ABIN2749126
  • Target Alle Fc gamma RII (CD32) Antikörper anzeigen
    Fc gamma RII (CD32)
    Reaktivität
    • 128
    • 11
    • 10
    • 10
    • 9
    • 9
    • 7
    • 3
    • 2
    • 1
    • 1
    Human
    Wirt
    • 72
    • 69
    Maus
    Klonalität
    • 78
    • 60
    • 3
    Monoklonal
    Konjugat
    • 49
    • 13
    • 11
    • 10
    • 5
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    PE
    Applikation
    • 81
    • 56
    • 23
    • 21
    • 13
    • 12
    • 11
    • 9
    • 7
    • 6
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Flow Cytometry (FACS)
    Spezifität
    The mouse monoclonal antibody 3D3 recognizes an extracellular epitope of CD32, a 40 kDa polymorphic transmembrane glycoprotein serving as the low affinity receptor for aggregated IgG. This antibody recognizes CD32 isoforms on B cells of all donors, but on platelets, monocytes, and granulocytes of only some donors (131R variant, but not 131H variant).
    Kreuzreaktivität (Details)
    Human
    Aufreinigung
    Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
    Immunogen
    purified glycosylated recombinant human FcgammaRIIa2
    Klon
    3D3
    Isotyp
    IgG1 kappa
    Top Product
    Discover our top product CD32 Primärantikörper
  • Applikationshinweise
    Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μL reagent / 100 μL of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
    Kommentare

    The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Buffer
    Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    4 °C
    Informationen zur Lagerung
    Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
  • Os, Bürgler, Ribes, Funderud, Wang, Thompson, Tjønnfjord, Bogen, Munthe: "Chronic lymphocytic leukemia cells are activated and proliferate in response to specific T helper cells." in: Cell reports, Vol. 4, Issue 3, pp. 566-77, (2013) (PubMed).

    Dai, Jayapal, Tay, Reghunathan, Lin, Too, Lim, Chan, Kemeny, Floto, Smith, Melendez, MacAry: "Differential signal transduction, membrane trafficking, and immune effector functions mediated by FcgammaRI versus FcgammaRIIa." in: Blood, Vol. 114, Issue 2, pp. 318-27, (2009) (PubMed).

    Dutertre, Bonnin-Gélizé, Pulford, Bourel, Fridman, Teillaud: "A novel subset of NK cells expressing high levels of inhibitory FcgammaRIIB modulating antibody-dependent function." in: Journal of leukocyte biology, Vol. 84, Issue 6, pp. 1511-20, (2008) (PubMed).

    Boruchov, Heller, Veri, Bonvini, Ravetch, Young: "Activating and inhibitory IgG Fc receptors on human DCs mediate opposing functions." in: The Journal of clinical investigation, Vol. 115, Issue 10, pp. 2914-23, (2005) (PubMed).

    Vely, Gruel, Moncuit, Cochet, Rouard, Dare, Galon, Sautes, Fridman, Teillaud: "A new set of monoclonal antibodies against human Fc gamma RII (CD32) and Fc gamma RIII (CD16): characterization and use in various assays." in: Hybridoma, Vol. 16, Issue 6, pp. 519-28, (1998) (PubMed).

  • Target
    Fc gamma RII (CD32)
    Andere Bezeichnung
    CD32 (CD32 Produkte)
    Synonyme
    CD32 antikoerper, CD32B antikoerper, FCG2 antikoerper, FCGR2 antikoerper, IGFR2 antikoerper, FCGR2B antikoerper, AI528646 antikoerper, F630109E10Rik antikoerper, Fc[g]RII antikoerper, FcgRII antikoerper, Fcgr2 antikoerper, Fcgr2a antikoerper, Fcr-2 antikoerper, Fcr-3 antikoerper, Ly-17 antikoerper, Ly-m20 antikoerper, LyM-1 antikoerper, Lym-1 antikoerper, FCRII antikoerper, FcRII antikoerper, Fc fragment of IgG receptor IIb antikoerper, Fc receptor, IgG, low affinity IIb antikoerper, FCGR2B antikoerper, Fcgr2b antikoerper
    Hintergrund
    Fc fragment of IgG receptor IIa,CD32 (FcgammaRII) is a low affinity receptor for aggregated IgG. It is strongly expressed on monocytes, granulocytes, myeloid and myeloblastic cell lines, and weakly on B cells, CD34+ bone marrow cells, and resting and activated platelets. After binding its ligand, CD32 induces IgG-mediated phagocytosis and oxidative burst in monocytes and neutrophils, whereas in B cells it mediates a negative signal. This polymorphic transmembrane glycoprotein is expressed not only in the activating (CD32a) and inhibitory isoform (CD32b), but also in individual variants with differing avidities for IgG subtypes (e.g. the CD32a131R and CD32a131H allotypes).,FCG2, FCGR2A, IGFR2
    Gen-ID
    2212
    UniProt
    P12318
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