IKZF2 Antikörper (AA 51-107) (PE)

Produktnummer ABIN2749147

Produktdetails anti-IKZF2 Antikörper (PE)

Target: IKZF2 (IKAROS Family Zinc Finger 2 (IKZF2))

Bindungsspezifität: AA 51-107

Reaktivität: Human, Maus

Wirt: Armenischer Hamster

Klonalität: Monoklonal

Konjugat: Dieser IKZF2 Antikörper ist konjugiert mit PE

Applikation: Flow Cytometry (FACS)

Spezifität: The Armenian hamster monoclonal antibody 22F6 recognizes Helios, a transcription factor (intracellular antigen) expressed in some hematopoietic stem cells, and at high levels in thymic-derived regulatory T cells. The epitope is located between amino acids 51 and 107.

Kreuzreaktivität (Details): Human, Mouse

Aufreinigung: Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Immunogen: Peptide coresponding to the amino acids 51-107 of Helios

Klon: 22F6

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μL reagent / 100 μL of whole blood or 10⁶ cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests. Intracellular staining.

Kommentare:

The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

Referenzen für anti-IKZF2 Antikörper (PE) (ABIN2749147)

Blankenhaus, Reitz, Brenz, Eschbach, Hartmann, Haben, Sparwasser, Huehn, Kühl, Feyerabend, Rodewald, Breloer: "Foxp3? regulatory T cells delay expulsion of intestinal nematodes by suppression of IL-9-driven mast cell activation in BALB/c but not in C57BL/6 mice." in: PLoS pathogens, Vol. 10, Issue 2, pp. e1003913, (2014) (PubMed).

Atarashi, Tanoue, Oshima, Suda, Nagano, Nishikawa, Fukuda, Saito, Narushima, Hase, Kim, Fritz, Wilmes, Ueha, Matsushima, Ohno, Olle, Sakaguchi, Taniguchi, Morita, Hattori, Honda: "Treg induction by a rationally selected mixture of Clostridia strains from the human microbiota." in: Nature, Vol. 500, Issue 7461, pp. 232-6, (2013) (PubMed).

McIver, Melenhorst, Wu, Grim, Ito, Cho, Hensel, Battiwalla, Barrett: "Donor lymphocyte count and thymic activity predict lymphocyte recovery and outcomes after matched-sibling hematopoietic stem cell transplant." in: Haematologica, Vol. 98, Issue 3, pp. 346-52, (2013) (PubMed).

Serre, Bénézech, Desanti, Bobat, Toellner, Bird, Chan, Kastner, Cunningham, Maclennan, Mohr: "Helios is associated with CD4 T cells differentiating to T helper 2 and follicular helper T cells in vivo independently of Foxp3 expression." in: PLoS ONE, Vol. 6, Issue 6, pp. e20731, (2011) (PubMed).

Pinheiro, Singh, Grant, Appleton, Sacchini, Walker, Chadbourne, Palmer, Armitage-Chan, Thompson, Williamson, Cunningham, Garden: "Phenotypic and functional characterization of a CD4(+) CD25(high) FOXP3(high) regulatory T-cell population in the dog." in: Immunology, Vol. 132, Issue 1, pp. 111-22, (2010) (PubMed).

Details zu IKZF2

Target: IKZF2 (IKAROS Family Zinc Finger 2 (IKZF2))

Andere Bezeichnung: Helios (IKZF2 Produkte)

Synonyme: ANF1A2 antikoerper, HELIOS antikoerper, ZNF1A2 antikoerper, ZNFN1A2 antikoerper, helios antikoerper, znf1a2 antikoerper, znfn1a1 antikoerper, znfn1a2 antikoerper, IKZF2 antikoerper, MGC159581 antikoerper, DKFZp469N0319 antikoerper, A730095J18Rik antikoerper, Helios antikoerper, Zfpn1a2 antikoerper, Znfn1a2 antikoerper, ikzf2 antikoerper, znfn1a1-a antikoerper, IKAROS family zinc finger 2 antikoerper, si:zfos-1425h8.1 antikoerper, IKAROS family zinc finger 2 (Helios) antikoerper, IKAROS family zinc finger 2 L homeolog antikoerper, IKZF2 antikoerper, ikzf2 antikoerper, Ikzf2 antikoerper, si:zfos-1425h8.1 antikoerper, ikzf2.L antikoerper

Hintergrund: IKAROS family zinc finger 2,Helios, also known as IKZF2 (Ikaros family zinc finger protein 2) is a hematopoietic-specific transcription factor involved in the regulation of lymphocyte development, together with other members of this family, such as Aiolos and Ikaros. Helios forms homo- and heterodimers with these proteins and is thought to function predominantly in early hematopoietic development. Expression of Helios, Aiolos and Ikaros is restricted to cells of the hematopoietic system, whereas other family members, Eos and Pegassus, are more widely expressed. Helios is expressed at early stages of thymocyte development. In mature T cells, Helios has been strongly associated with Treg cells.,ANF1A2, IKZF2

Gen-ID: 22807

UniProt: Q9UKS7