FAS Antikörper

Produktnummer ABIN2749190

Produktdetails anti-FAS Antikörper

Target: FAS (TNF Receptor Superfamily, Member 6 (FAS))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser FAS Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Functional Studies (Func)

Spezifität: The mouse monoclonal antibody EOS9.1 recognizes an extracellular epitope of CD95 (Fas/APO-1), a 46 kDa glycoprotein of the tumour necrosis factor/nerve growth factor (TNF/NGF) receptor superfamily, expressed on a variety of normal and neoplastic cells.

Kreuzreaktivität (Details): Human

Aufreinigung: Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods).

Reinheit: > 95 % (by SDS-PAGE)

Endotoxin-Niveau: Endotoxin level is less than 0.01 EU/µg of the protein, as determined by the LAL test.

Immunogen: P815 cells transfected with human CD95

Klon: EOS9-1

Isotyp: IgM kappa

Anwendungsinformationen

Applikationshinweise: Functional application: In vitro induction of apoptosis.
Flow cytometry: Recommended dilution: 2-6 μg/mL.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4

Konservierungsmittel: Azide free

Lagerung: 4 °C

Informationen zur Lagerung: Store at 2-8°C. Do not freeze.

Referenzen für anti-FAS Antikörper (ABIN2749190)

Matsuoka, Kim, McDonough, Bascug, Warshauer, Koreth, Cutler, Ho, Alyea, Antin, Soiffer, Ritz: "Altered regulatory T cell homeostasis in patients with CD4+ lymphopenia following allogeneic hematopoietic stem cell transplantation." in: The Journal of clinical investigation, Vol. 120, Issue 5, pp. 1479-93, (2010) (PubMed).

Kasper, Konze, Kern, Stippel: "CD95 and TNFα-induced apoptosis in liver metastases of colorectal carcinoma." in: In vivo (Athens, Greece), Vol. 24, Issue 5, pp. 653-7, (2010) (PubMed).

Conejo-Garcia, Benencia, Courreges, Gimotty, Khang, Buckanovich, Frauwirth, Zhang, Katsaros, Thompson, Levine, Coukos: "Ovarian carcinoma expresses the NKG2D ligand Letal and promotes the survival and expansion of CD28- antitumor T cells." in: Cancer research, Vol. 64, Issue 6, pp. 2175-82, (2004) (PubMed).

Desbarats, Birge, Mimouni-Rongy, Weinstein, Palerme, Newell: "Fas engagement induces neurite growth through ERK activation and p35 upregulation." in: Nature cell biology, Vol. 5, Issue 2, pp. 118-25, (2003) (PubMed).

Details zu FAS

Target: FAS (TNF Receptor Superfamily, Member 6 (FAS))

Andere Bezeichnung: CD95 / Fas (FAS Produkte)

Synonyme: ALPS1A antikoerper, APO-1 antikoerper, APT1 antikoerper, CD95 antikoerper, FAS1 antikoerper, FASTM antikoerper, TNFRSF6 antikoerper, AI196731 antikoerper, APO1 antikoerper, TNFR6 antikoerper, Tnfrsf6 antikoerper, lpr antikoerper, Fas cell surface death receptor antikoerper, Fas (TNF receptor superfamily member 6) antikoerper, FAS antikoerper, Fas antikoerper, fas antikoerper

Hintergrund: Fas cell surface death receptor,CD95 (Fas, APO-1), a 46 kDa transmembrane glycoprotein, is a cell death receptor of the TNFR superfamily. Stimulation of CD95 results in aggregation of its intracellular death domains, formation of the death-inducing signaling complex (DISC) and activation of caspases. In type I cells caspase 3 is activated by high amounts of caspase 8 generated at the DISC, in type II cells low concentration of caspase 8 activates pathway leading to the release of cytochrome c from mitochondria and activation of caspase 3 by cytochom c. Besides its roles in induction of apoptosis, Fas also triggers pro-inflammatory cytokine responses.,FAS1, APT1, APO-1, FASTM, ALPS1A, TNFRSF6

Gen-ID: 355

UniProt: P25445

Pathways: p53 Signalweg, Apoptose, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity