NEDD4-2 Antikörper (Middle Region)

Produktnummer ABIN2774731

Produktdetails anti-NEDD4-2 Antikörper

Target: NEDD4-2 (NEDD4L) (E3 ubiquitin-protein ligase NEDD4-like (NEDD4L))

Bindungsspezifität: Middle Region

Reaktivität: Human, Maus, Ratte, Rind (Kuh), Hund, Meerschweinchen, Pferd, Kaninchen, Zebrafisch (Danio rerio)

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NEDD4-2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Sequenz: TVTLSAPLEG AKDSPVRRAV KDTLSNPQSP QPSPYNSPKP QHKVTQSFLP

Homologie: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 93%

Produktmerkmale: This is a rabbit polyclonal antibody against NEDD4L. It was validated on Western Blot using a cell lysate as a positive control.

Aufreinigung: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human NEDD4L

Anwendungsinformationen

Applikationshinweise: Optimal working dilutions should be determined experimentally by the investigator.

Kommentare:

Antigen size: 955 AA

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Referenzen für anti-NEDD4-2 Antikörper (ABIN2774731)

Raikwar, Thomas: "Nedd4-2 isoforms ubiquitinate individual epithelial sodium channel subunits and reduce surface expression and function of the epithelial sodium channel." in: American journal of physiology. Renal physiology, Vol. 294, Issue 5, pp. F1157-65, (2008) (PubMed).

Details zu NEDD4-2

Target: NEDD4-2 (NEDD4L) (E3 ubiquitin-protein ligase NEDD4-like (NEDD4L))

Andere Bezeichnung: NEDD4L (NEDD4L Produkte)

Synonyme: NEDD4-2 antikoerper, NEDD4.2 antikoerper, RSP5 antikoerper, hNEDD4-2 antikoerper, nedd4 antikoerper, nedd4-2 antikoerper, NEDD4 antikoerper, Nedd4-2 antikoerper, 1300012C07Rik antikoerper, Nedd4b antikoerper, neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase antikoerper, NEDD4L antikoerper, neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase S homeolog antikoerper, neural precursor cell expressed, developmentally down-regulated gene 4-like antikoerper, NEDD4L antikoerper, LOC776799 antikoerper, nedd4l.S antikoerper, Nedd4l antikoerper

Hintergrund: NEDD4L is an E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. NEDD4L inhibits TGF-beta signaling by triggering SMAD2 and TGFR1 ubiquitination and proteasome-dependent degradation. NEDD4L promotes ubiquitination and internalization of various plasma membrane channels such as ENaC, Nav1.2, Nav1.3, Nav1.5, Nav1.7, Nav1.8, Kv1.3, EAAT1 or CLC5. NEDD4L also promotes ubiquitination and degradation of SGK.
Alias Symbols: FLJ33870, KIAA0439, RSP5, hNedd4-2, NEDD4-2, NEDD4.2, hNEDD4-2
Protein Interaction Partner: NEDD4L, DAZAP2, UBE2D1, PTCH1, WNK3, TNK2, SNCA, SLC9A3, SMAD6, KCNH2, HCN1, SMAD3, UBE2N, SNA3, NOTCH1, UBE2D3, UBC, SGK1, KCNQ1, SGK3, GORAB, MED28, FBXL15, vpr, RNF11, AATF, YWHAQ, LAPTM5, SCNN1B, SMAD2, ARRDC3, ARRDC4, UBE2L3, UBE2D2, SMAD7, UBE2K, UB
Protein Size: 955

Molekulargewicht: 110 kDa

Gen-ID: 23327

NCBI Accession: NM_015277, NP_056092

Pathways: Negative Regulation of Transporter Activity