CHFR Antikörper (N-Term)

Produktnummer ABIN2774757

Produktdetails anti-CHFR Antikörper

Target: CHFR (Checkpoint with Forkhead and Ring Finger Domains (CHFR))

Bindungsspezifität: N-Term

Reaktivität: Human, Maus, Ratte, Kaninchen, Zebrafisch (Danio rerio), Pferd, Rind (Kuh), Hund, Meerschweinchen

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CHFR Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Sequenz: REWTIGRRRG CDLSFPSNKL VSGDHCRIVV DEKSGQVTLE DTSTSGTVIN

Homologie: Cow: 93%, Dog: 93%, Guinea Pig: 100%, Horse: 93%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 86%

Produktmerkmale: This is a rabbit polyclonal antibody against CHFR. It was validated on Western Blot using a cell lysate as a positive control.

Aufreinigung: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human CHFR

Anwendungsinformationen

Applikationshinweise: Optimal working dilutions should be determined experimentally by the investigator.

Kommentare:

Antigen size: 623 AA

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Details zu CHFR

Target: CHFR (Checkpoint with Forkhead and Ring Finger Domains (CHFR))

Andere Bezeichnung: CHFR (CHFR Produkte)

Synonyme: RNF116 antikoerper, RNF196 antikoerper, CHFR antikoerper, fc43f10 antikoerper, si:dkey-69h6.7 antikoerper, wu:fc43f10 antikoerper, rnf116 antikoerper, rnf196 antikoerper, 5730484M20Rik antikoerper, C230082M18 antikoerper, checkpoint with forkhead and ring finger domains antikoerper, checkpoint with forkhead and ring finger domains, E3 ubiquitin protein ligase antikoerper, checkpoint with forkhead and ring finger domains, E3 ubiquitin protein ligase L homeolog antikoerper, CHFR antikoerper, chfr antikoerper, Chfr antikoerper, chfr.L antikoerper

Hintergrund: CHFR is an E3 ubiquitin-protein ligase required to transiently arrest cells in early prophase when they are exposed to microtubule poisons. It acts in early prophase before chromosome condensation, when the centrosome moves apart from each other along the periphery of the nucleus. CHFR probably promotes the formation of 'Lys-63'-linked polyubiquitin chains and functions with the specific ubiquitin-conjugating UBC13-MMS2 (UBE2N-UBE2V2) heterodimer. Substrates that are polyubiquitinated at 'Lys-63' are usually not targeted for degradation, but are rather involved in signaling cellular stress. This suggests that it may be involved in signaling the presence of mitotic stress caused by microtubule poisons.
Alias Symbols: FLJ10796, FLJ33629, RNF116, RNF196
Protein Interaction Partner: USP7, VIM, UBE2D2, AURKA, HLTF, RANBP2, PLK1, PCNA, MCM2, HDAC2, HDAC1, CDK5, CDC20, PARP1, SIN3A, CHFR, UBC13, SUMO1, UBE2I, LRP1, UBE2V1, UBE2N, UBE2D1, TRIM9, RNF125, CBLC, RNF7, RELA, RNF8, HIST2H2BE, HIST2H2AC, UBC, SMARCD1, SMARCB1, SMARCA4, KIF22,
Protein Size: 623

Molekulargewicht: 69 kDa

Gen-ID: 55743

NCBI Accession: NM_018223, NP_060693

UniProt: Q96EP1