ube3a Antikörper (Middle Region)

Produktnummer ABIN2775648

Produktdetails anti-ube3a Antikörper

Target: ube3a (Ubiquitin Protein Ligase E3A (ube3a))

Bindungsspezifität: Middle Region

Reaktivität: Human, Maus, Ratte, Kaninchen, Hund, Meerschweinchen, Pferd, Zebrafisch (Danio rerio), Rind (Kuh)

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ube3a Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Sequenz: AKNGPDTERL PTSHTCFNVL LLPEYSSKEK LKERLLKAIT YAKGFGML

Homologie: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 86%

Produktmerkmale: This is a rabbit polyclonal antibody against UBE3A. It was validated on Western Blot using a cell lysate as a positive control.

Aufreinigung: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human UBE3A

Anwendungsinformationen

Applikationshinweise: Optimal working dilutions should be determined experimentally by the investigator.

Kommentare:

Antigen size: 875 AA

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Details zu ube3a

Target: ube3a (Ubiquitin Protein Ligase E3A (ube3a))

Andere Bezeichnung: UBE3A (ube3a Produkte)

Hintergrund: UBE3A is an E3 ubiquitin-protein ligase, part of the ubiquitin protein degradation system. This imprinted gene is maternally expressed in brain and biallelically expressed in other tissues. Maternally inherited deletion of this gene causes Angelman Syndrome, characterized by severe motor and intellectual retardation, ataxia, hypotonia, epilepsy, absence of speech, and characteristic facies. The protein also interacts with the E6 protein of human papillomavirus types 16 and 18, resulting in ubiquitination and proteolysis of tumor protein p53.Western blots using two different antibodies against two unique regions of this protein target confirm the same apparent molecular weight in our tests.This gene encodes an E3 ubiquitin-protein ligase, part of the ubiquitin protein degradation system. This imprinted gene is maternally expressed in brain and biallelically expressed in other tissues. Maternally inherited deletion of this gene causes Angelman Syndrome, characterized by severe motor and intellectual retardation, ataxia, hypotonia, epilepsy, absence of speech, and characteristic facies. The protein also interacts with the E6 protein of human papillomavirus types 16 and 18, resulting in ubiquitination and proteolysis of tumor protein p53. Alternative splicing of this gene results in three transcript variants encoding three isoforms with different N-termini. Additional transcript variants have been described, but their full length nature has not been determined.
Alias Symbols: ANCR, AS, E6-AP, EPVE6AP, FLJ26981, HPVE6A
Protein Interaction Partner: KLHL38, UBE2D1, UBB, TP53, TAT, AFG3L1P, ATG9A, ASAP3, AHSP, UBE3A, UBC, MEOX2, MAGEA8, JADE1, YOD1, UBE2L3, UBE2D3, PSMD14, SHFM1, ARNTL, LPP, HSF1, DR1, BTF3L4, LRSAM1, P3H1, NPLOC4, IPO11, DBNL, LSM1, ARPC1B, METTL1, SACS, ARC, RAD23A, PTPN3, CEBPA, SC
Protein Size: 875

Molekulargewicht: 101 kDa

Gen-ID: 7337

NCBI Accession: NM_000462, NP_000453

UniProt: Q05086

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway