MED27 Antikörper (Middle Region)

Produktnummer ABIN2778099

Produktdetails anti-MED27 Antikörper

Target: MED27 (Mediator Complex Subunit 27 (MED27))

Bindungsspezifität: Middle Region

Reaktivität: Human, Maus, Rind (Kuh), Hund, Ratte, Meerschweinchen, Pferd, Kaninchen

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser MED27 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Sequenz: LSRPNGTSAM LLVTLGKVLK VIVVMRSLFI DRTIVKGYNE NVYTEDGKLD

Homologie: Cow: 100%, Dog: 85%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 92%, Rabbit: 85%, Rat: 100%

Produktmerkmale: This is a rabbit polyclonal antibody against MED27. It was validated on Western Blot using a cell lysate as a positive control.

Aufreinigung: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human MED27

Anwendungsinformationen

Applikationshinweise: Optimal working dilutions should be determined experimentally by the investigator.

Kommentare:

Antigen size: 311 AA

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Details zu MED27

Target: MED27 (Mediator Complex Subunit 27 (MED27))

Andere Bezeichnung: MED27 (MED27 Produkte)

Hintergrund: The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. MED27 is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors.The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors.
Alias Symbols: CRAP34, CRSP34, CRSP8, MGC11274, TRAP37
Protein Interaction Partner: UBC, CDK19, CDK8, MED19, ARFGAP1, MED26, SOX5, POLR2C, RBPJ, AFP, FBXW7, EPAS1, MED4, MED12, MED14, MED1, MED28, OBFC1, MED18, ZC3H13, QKI, TRIP4, TADA2A, MED9, MED30, MED8, MED22, MED10, MED29,
Protein Size: 311

Molekulargewicht: 35 kDa

Gen-ID: 9442

NCBI Accession: NM_004269, NP_004260

UniProt: Q6P2C8

Pathways: Stem Cell Maintenance, Regulation of Lipid Metabolism by PPARalpha