SRSF1 Antikörper (C-Term)

Produktnummer ABIN2779013

Produktdetails anti-SRSF1 Antikörper

Target: SRSF1 (serine/arginine-Rich Splicing Factor 1 (SRSF1))

Bindungsspezifität: C-Term

Reaktivität: Human, Maus, Ratte, Hund, Zebrafisch (Danio rerio), Meerschweinchen, Rind (Kuh), Pferd

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SRSF1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Sequenz: EFVRKEDMTY AVRKLDNTKF RSHEGETAYI RVKVDGPRSP SYGRSRSRSR

Homologie: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rat: 100%, Zebrafish: 100%

Produktmerkmale: This is a rabbit polyclonal antibody against SFRS1. It was validated on Western Blot using a cell lysate as a positive control.

Aufreinigung: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human SFRS1

Anwendungsinformationen

Applikationshinweise: Optimal working dilutions should be determined experimentally by the investigator.

Kommentare:

Antigen size: 248 AA

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Referenzen für anti-SRSF1 Antikörper (ABIN2779013)

Li, Xia, Huang, Chen, Su, Li, Wang, Ding, Shao: "A strategy to rapidly identify the functional targets of microRNAs by combining bioinformatics and mRNA cytoplasmic/nucleic ratios in culture cells." in: FEBS letters, Vol. 584, Issue 14, pp. 3198-202, (2010) (PubMed).

Details zu SRSF1

Target: SRSF1 (serine/arginine-Rich Splicing Factor 1 (SRSF1))

Andere Bezeichnung: SFRS1 (SRSF1 Produkte)

Synonyme: ASF antikoerper, SF2 antikoerper, SF2p33 antikoerper, SFRS1 antikoerper, SRp30a antikoerper, 1110054N12Rik antikoerper, 5730507C05Rik antikoerper, 6330415C05Rik antikoerper, AI482334 antikoerper, AW491331 antikoerper, Asf antikoerper, Sf2 antikoerper, Sfrs1 antikoerper, DKFZp469K2419 antikoerper, sfrs1 antikoerper, sfrs1b antikoerper, wu:fb80g05 antikoerper, zgc:111894 antikoerper, zgc:65898 antikoerper, zgc:76897 antikoerper, asf antikoerper, sf2 antikoerper, sf2p33 antikoerper, srp30a antikoerper, sfrs1a antikoerper, sfrs1l antikoerper, wu:fb52g10 antikoerper, wu:fb97g12 antikoerper, zgc:66146 antikoerper, serine and arginine rich splicing factor 1 antikoerper, serine/arginine-rich splicing factor 1 antikoerper, serine/arginine-rich splicing factor 1b antikoerper, serine/arginine-rich splicing factor 1 L homeolog antikoerper, serine/arginine-rich splicing factor 1a antikoerper, SRSF1 antikoerper, Srsf1 antikoerper, srsf1b antikoerper, srsf1.L antikoerper, srsf1a antikoerper

Hintergrund: SFRS1 is a member of the arginine/serine-rich splicing factor protein family, and functions in both constitutive and alternative pre-mRNA splicing. The protein binds to pre-mRNA transcripts and components of the spliceosome, and can either activate or repress splicing depending on the location of the pre-mRNA binding site. The protein's ability to activate splicing is regulated by phosphorylation and interactions with other splicing factor associated proteins. Multiple transcript variants encoding different isoforms have been found for this gene.Alternative mRNA splicing plays an important role in development and differentiation, many transcripts are spliced differently in distinct cell types and tissues. Both constitutive and alternative splicing occurs on spliceosomes, which are complex particles composed of small nuclear ribonucleoproteins (snRNPs) and non-snRNP proteins. The SR family of non-snRNP splicing factors is characterized by the presence of an RNA recognition motif and a serine- and arginine-rich (SR) domain. SR proteins are required at early stages of spliceosome assembly, have distinct but overlapping specificities for different pre-mRNAs, and can alter splice site choice, suggesting that they may be involved in the regulation of alternative splicing in vivo. Two of the SR proteins, ASF/SF2 (SFRS1) and SC35 (SFRS2, MIM 600813), have been extensively characterized.Alternative mRNA splicing plays an important role in development and differentiation, many transcripts are spliced differently in distinct cell types and tissues. Both constitutive and alternative splicing occurs on spliceosomes, which are complex particles composed of small nuclear ribonucleoproteins (snRNPs) and non-snRNP proteins. The SR family of non-snRNP splicing factors is characterized by the presence of an RNA recognition motif and a serine- and arginine-rich (SR) domain. SR proteins are required at early stages of spliceosome assembly, have distinct but overlapping specificities for different pre-mRNAs, and can alter splice site choice, suggesting that they may be involved in the regulation of alternative splicing in vivo. Two of the SR proteins, ASF/SF2 (SFRS1) and SC35 (SFRS2, MIM 600813), have been extensively characterized (Bermingham et al., 1995).[supplied by OMIM].
Alias Symbols: ASF, SF2, SFRS1, SF2p33, SRp30a, SRSF1
Protein Interaction Partner: TRAF3IP2, HUWE1, SPRTN, STAU1, UBC, MDM2, ZBTB1, RNF2, EZH2, EED, SUZ12, PARK2, SRPK2, SRPK1, SRPK3, FBXO6, YWHAE, U2AF2, PAN2, MPG, CIR1, FN1, CDK6, HNRNPA0, RNPS1, SRSF10, NXF1, PRPF4, BCL10, U2AF1, SRSF4, MAGOH, SMURF1, EIF4A3, ESR1, ECT2, BRCA1, BARD1
Protein Size: 248

Molekulargewicht: 27 kDa

Gen-ID: 6426

NCBI Accession: NM_006924, NP_008855

UniProt: Q07955

Pathways: Ribonucleoprotein Complex Subunit Organization