SMAD2 Antikörper (N-Term)

Produktnummer ABIN2779412

Der Kaninchen Polyklonal Anti-SMAD2-Antikörper wurde für WB und IHC validiert. Er ist geeignet, SMAD2 in Proben von Human, Maus, Ratte, Rind (Kuh), Kaninchen, Hund, Pferd und Meerschweinchen zu detektieren.

Kurzübersicht für SMAD2 Antikörper (N-Term) (ABIN2779412)

Target: SMAD2 (SMAD, Mothers Against DPP Homolog 2 (SMAD2))

Reaktivität: Human, Maus, Ratte, Rind (Kuh), Kaninchen, Hund, Pferd, Meerschweinchen

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SMAD2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Produktdetails anti-SMAD2 Antikörper

Bindungsspezifität: N-Term

Sequenz: MSSILPFTPP VVKRLLGWKK SAGGSGGAGG GEQNGQEEKW CEKAVKSLVK

Homologie: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%

Produktmerkmale: This is a rabbit polyclonal antibody against SMAD2. It was validated on Western Blot using a cell lysate as a positive control.

Aufreinigung: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human SMAD2

Anwendungsinformationen

Applikationshinweise: Optimal working dilutions should be determined experimentally by the investigator.

Kommentare:

Antigen size: 467 AA

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Details zu SMAD2

Target: SMAD2 (SMAD, Mothers Against DPP Homolog 2 (SMAD2))

Andere Bezeichnung: SMAD2

Hintergrund: SMAD2 belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. SMAD2 mediates the signal of the transforming growth factor (TGF)-beta, and thus regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. SMAD2 is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein. In response to TGF-beta signal, SMAD2 is phosphorylated by the TGF-beta receptors. The phosphorylation induces the dissociation of this protein with SARA and the association with the family member SMAD4. The association with SMAD4 is important for the translocation of this protein into the nucleus, where it binds to target promoters and forms a transcription repressor complex with other cofactors. This protein can also be phosphorylated by activin type 1 receptor kinase, and mediates the signal from the activin.The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signal of the transforming growth factor (TGF)-beta, and thus regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. This protein is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein. In response to TGF-beta signal, this protein is phosphorylated by the TGF-beta receptors. The phosphorylation induces the dissociation of this protein with SARA and the association with the family member SMAD4. The association with SMAD4 is important for the translocation of this protein into the nucleus, where it binds to target promoters and forms a transcription repressor complex with other cofactors. This protein can also be phosphorylated by activin type 1 receptor kinase, and mediates the signal from the activin. Alternatively spliced transcript variants encoding the same protein have been observed.
Alias Symbols: JV18, JV18-1, MADH2, MADR2, MGC22139, MGC34440, hMAD-2, hSMAD2
Protein Interaction Partner: HDAC2, UBC, SMAD4, SKIL, ANP32B, TUBA1B, SYT1, SOD1, ANP32E, PTMS, NEFM, JUN, ATP5I, RHOA, OTUB1, ZFYVE9, PPARG, TP53, TGFBR1, TRIM33, HCVgp1, TSC2, FHL3, FHL2, FHL1, CSNK1D, KLF5, SH2D2A, SKI, LCK, IRF7, IRF3, RNF111, LEMD3, PIK3CA, ST13, ST5, SRI, SPTBN
Protein Size: 467

Molekulargewicht: 52 kDa

Gen-ID: 4087

NCBI Accession: NM_005901, NP_005892

UniProt: Q15796

Pathways: Zellzyklus, Hormone Transport, Chromatin Binding, Protein targeting to Nucleus