NP (Middle Region) Antikörper

Produktnummer ABIN405995

Produktdetails

Target: NP

Bindungsspezifität: Middle Region

Reaktivität: Human, Maus, Ratte, Rind (Kuh), Kaninchen, Saccharomyces cerevisiae, Hund, Zebrafisch (Danio rerio), Meerschweinchen, Pferd

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Unkonjugiert

Applikation: Western Blotting (WB)

Sequenz: GVDTLVVTNA AGGLNPKFEV GDIMLIRDHI NLPGFSGQNP LRGPNDERFG

Homologie: Cow: 86%, Dog: 86%, Guinea Pig: 93%, Horse: 93%, Human: 100%, Mouse: 93%, Rabbit: 86%, Rat: 93%, Yeast: 91%, Zebrafish: 79%

Produktmerkmale: This is a rabbit polyclonal antibody against NP. It was validated on Western Blot using a cell lysate as a positive control.

Aufreinigung: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human NP

Anwendungsinformationen

Applikationshinweise: Optimal working dilutions should be determined experimentally by the investigator.

Kommentare:

Antigen size: 289 AA

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Referenzen

Ghanem, Li, Wing, Schramm: "Altered thermodynamics from remote mutations altering human toward bovine purine nucleoside phosphorylase." in: Biochemistry, Vol. 47, Issue 8, pp. 2559-64, (2008) (PubMed).

Antigendetails

Target: NP

Substanzklasse: Viral Protein

Hintergrund: Defects in NP are the cause of nucleoside phosphorylase deficiency (NP deficiency). It leads to a severe T-cell immunodeficiency with neurologic disorder in children. The specific function of NP is not yet known.This gene encodes an enzyme which reversibly catalyzes the phosphorolysis of purine nucleosides. The enzyme is trimeric, containing three identical subunits. Mutations which result in nucleoside phosphorylase deficiency result in defective T-cell (cell-mediated) immunity but can also affect B-cell immunity and antibody responses. Neurologic disorders may also be apparent in patients with immune defects. A known polymorphism at aa position 51 that does not affect enzyme activity has been described. A pseudogene has been identified on chromosome 2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: MGC117396, MGC125915, MGC125916, PNP, PRO1837, PUNP, NP
Protein Interaction Partner: PNP, UBC, SUMO2, PPME1, SBDS, PSAT1, GMPS, RAB1A, PSMD5, MTAP, ENO2, HECW2, ITGA4, NADK2, TMEM177, DNAJB11, NDUFV1, GLO1, UBD, CDK2, SIRT7, UCHL5, LRIF1, CCDC90B, RBM48, ZHX1, TFB2M, NCALD, TFAM, TP53, APLP1,
Protein Size: 289

Molekulargewicht: 32 kDa

Gen-ID: 4860

NCBI Accession: NM_000270, NP_000261

UniProt: P00491